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Powering Your Discoveries
We are committed to advancing protein and cellular analysis through innovative instruments that provide enhanced accuracy, speed, and sensitivity.
Our tools are designed for precise protein charge characterization, purity analysis, accurate protein quantitation, measuring post-translational modifications, and sterile single-cell sorting.
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Cell Sorting and Single Cell Dispensing Ready for fast, gentle, and easy single cell isolation at your fingertips? The Pala instrument performs effortless sorting and dispensing of single cells in one step for a wide range of applications.
Automated ELISA Rethink your ELISA with Ella, a fully automated quantitative immunoassay system that delivers fast, hands- free, reproducible data across multiple users and sites.
Biomolecular Characterization Need information on protein purity, identity, charge heterogeneity, and charge heterogeneity fractions for characterization, all in a day? Meet the Maurice Family: Maurice and MauriceFlex.
Meet Our Instruments Scan the QR Code or Visit: bio-techne.com/instruments
Meet Our Cell & Protein Characterization Problem Solvers
Automated Capillary Western Analysis Need protein quantity, identity, expression, or isoform data from 3 µL of cell or tissue lysates in as little as 3 hours? Look no further than Simple Western Technology, with Jess, Abby, and Leo Systems.
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M eet Pala
Extremely low fluid pressure to ensure the viability and function of cells
Broad range of sample cell concentrations 1000 cells/mL – 11x106 cells/mL
Easy to operate with no calibration and low maintenance
Separation of single cells into 96-well plates in less than 2 minutes, and separation into 384-well plates in less than 6 minutes
Disposable cell sorting cartridge avoids the risk of cross contamination
Lightweight, compact and portable, fits inside your cell culture hood
Pala Advantages
Flexible Single Cell Sorting Pala is a flexible benchtop cell sorter and single cell dispenser that combines innovative microfluidics, flow cytometry, and liquid dispensing technologies to screen and separate individual cells into 96- or 384-well plates in minutes.
Low system pressure (<2 psi) ensures gentle sorting and maintains cell viability and integrity. The innovative disposable cell sorting cartridge avoids the risk of cross contamination among samples, ensures sterility, and makes the running of multiple samples or cell types faster and easier.
Meet Pala Scan the QR Code or Visit: bio-techne.com/pala
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Discover More with Pala Accelerate Your Cell Line Development Workflow Pala single cell dispenser offers an automated, high-throughput alternative to traditional single cell isolation methods such as FACS sorting. The rapid and gentle single cell isolation offered by our benchtop instrument can help to preserve cell integrity and viability.
Colony Outgrowth Compared to Limiting Dilution A comparison of the Pala and limiting dilution show equivalent outgrowth across multiple cell types, highlighting the gentleness of the Pala.
D A T A N O T E S :
Single Cell Dispensing Accuracy Using a variety of cell types, the Pala proved highly accurate, efficient, and consistent at dispensing single cells compared to limiting dilution.
Sorting Pressure
Start-Up Time
Fluorescence Channels
Ease of Use
Minimal Cell Input
Sheath
Droplet Size
Sterile Sorting
Plate Based Sorting
Cost
Ideal for
Less than 2psi
5 minutes
Up to 11
Simple
100 cells
Water or PBS (6.5mL/hour)
1µL
Easy
96/384 well plates
Pala
$
Single cell cloning, Sterile sorting, All lab users
20–70psi
More than 45 minutes
16
Complex
More than 100,000 cells
FACS Buffer (1–2L/hour)
Nanoliter
Difficult
Optional
FACS
$$$$
Bulk sorting large cell numbers, Cell analysis, Expert lab users
T E C H N I C A L S P E C S
Figure 01A and 01B. Three cell types (HEK293, CHO, and A549) were dispensed with Pala as single cells comprising 70 x 96-well plates split between 6 instruments. Colony outgrowth was calculated as a % of single cells that grew into colonies. Colony outgrowth rates were nearly identical between Pala dispensed and hand pipetted cells indicating that Pala has no additional detrimental impact on cell survival compared to hand pipetting.
F I G U R E // 0 1 A
Pala Single Cell Deposition Accuracy by Cell Type
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Cell Type
HEK293 CHO A549 Limiting Dilution (Avg.)
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F I G U R E // 0 1 B
Outgrowth Efficiency: Pala vs. Limiting Dilution
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Cell Type & Deposition Method (Pala vs LD)
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estern
Simple Western Advantages Fast and Fully automated western workflow
Analyze up to 96 samples in just 3 hours
Highly reproducible and quantitative data offering <20% intra and inter assay CVs
3–4 log dynamic range with picogram-level sensitivity
Use as little as 3µL of sample
Multiplex and Reprobing capable
Normalize to total protein in the same run
Automated Capillary Western Analysis Elevate your protein analysis with Simple Western™ Technology, a cutting-edge solution that automates and simplifies the traditional western blot process. By integrating protein separation and immunodetection into a fully automated capillary system, it delivers precise, reproducible, and quantitative data without the hassle of manual steps. Simply load your samples and reagents into the microplate and return in just 3 hours to fully analyzed, quantitated results—eliminating the time-consuming, error-prone tasks of traditional western blotting.
Meet Simple Western Scan the QR Code or Visit: bio-techne.com/simplewestern
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F I G U R E // 02 B
Target Protein Detection F I G U R E // 02 C
Total Protein Detection
Figure 02A. Electropherogram view of size based protein detection. Figure O2B. Virtual lane view of target protein detection with quantitative results table.
Figure O2C. Total protein detection. Decreasing concentrations of DnaK in HeLa lysate and negative controls (15, 7.5 & 3.75 μg/mL).
AbbyJessLeo
T E C H N I C A L S P E C S
2525100Max Capillaries Per Run
<3 hours<3 hours<3 hoursRun Time
3µL/well3µL/well3µL/wellMinimal Sample Volume Required
ChemiluminescenceChemiluminescence, Fluorescence
Chemiluminescence; Upgradeable to Fluorescence
Detection
YesYesYesRePlex™ (Strip & ReProbe Replacement)
Molecular weight ladder ranges from 2–440 kDaSize Range
Low pgLow pgLow pgChemiluminescence Immunoassay Sensitivity
3-4 logs3-4 logs3-4 logsChemiluminescence Immunoassay Dynamic Range
F I G U R E // 02 A
Size Based Protein Detection Discover More with Simple Western Systems
2,000
1,500
1,000
500
0 12 40 66 90 116 180
C he
m ilu
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MW (kDa)
pERK2 + ppERK2
pERK1 + ppERK1
3,000
2,500
D A T A N O T E S :
What data can you get? Quantitative results such as molecular weight, signal intensity (area), % area, and signal-to-noise for each immunodetected protein are presented in the results table automatically.
Simple Western data is displayed by lane in a virtual-blot like image similar to traditional results with one big exception—not only do you get more information, you get it as soon as the assay is complete.
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M eet Ella
Ella Advantages Fully automated, 90-minute ELISA
Highly sensitive, wide dynamic range
Highly reproducible data across multiple users and sites
No specialized training required
Customizable analyte configurations
Precise, reproducible results
Low sample requirement (25 μL)
Fully Automated Quantitative Immunoassays Ella is a next-generation benchtop automated ELISA platform designed to deliver accurate, reproducible data with no manual steps.
Utilizing a unique microfluidic design, the Ella™ Platform’s Simple Plex™ Assays are fully validated, highly sensitive and can analyze up to 8 analytes simultaneously in less than 90 minutes. The simple workflow and precisely controlled automation helps ensure consistent results no matter the lab, instrument, or operator.
Meet Ella Scan the QR Code or Visit: bio-techne.com/ella
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F I G U R E // 03 B
Ella Assay Fluorescent Detection Sensitivity
Discover More with Ella
Figure 03A. Testing across 3 sites, 11 users and 9 Ella instruments. 4 different assays were processed for CCL2, IL-6, TNF alpha and VEGF-A. Eight unique serum samples with 2 controls for a total of 704. CV of 7.5% was observed showing highly consistent and precise results across users, sites, and instruments.
Figure 03B. Ella uses a fluorescent readout allowing for 4-5 logs of dynamic range and lower limit of quantification (LLOQ) compared to traditional ELISA.
Sample Volume
Number of Analytes for Multiplexing
Plate Size
Manual Operation Duration
Whole Experiment Duration
Sensitivity
Experimental Precision
Experimental Dynamic Range
25µL
Up to 8
16 samples, 32 samples, 48 samples, and 72 samples
15 minutes
90 minutes, including sample dilution, and processing to generation of data analysis report
sub pg/mL
<10%
4–5 logs
Ella
T E C H N I C A L S P E C S
F I G U R E // 03A
Multi-Site Test (All Results Histogram)
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1.5
1.3
1.1
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CV = 7.5%
High Sensitivity Simple Plex assays on Ella utilize a fluorescent detection system to deliver excellent low-end sensitivity to detect low expressing proteins. This allows samples containing high and low abundance of analyte to be assayed at a common dilution, reducing the need to rerun samples or perform dilution series.
D A T A N O T E S :
Superior Reproducibility Precision The Ella platform’s hands-free automation eliminates sources of variability to deliver exceptional precision and reproducibility. The simple load-and-go workflow, built in standard curves, and precisely controlled automation help ensure consistent results no matter the lab, instrument, or operator.
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100
10
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0.1
10
1
0.1
0.01
Samples measured on Simple Plex
Samples measured on ELISA
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M eet M
aurice
Maurice Family Advantages
Analyze a variety of molecules including mAbs, AAVs, IgMs, Lentiviruses and more
Obtain high resolution, high throughput protein size separation
Get data in as little as 5.5 minutes
Maintain regulatory compliance with industry-approved software
Decrease your footprint by doing away with acrylamide
Use across stages: discovery to QC
Automated Protein Charge & Size Analysis Maurice Platforms are your one-stop instrument for automated icIEF (imaged capillary IsoElectric Focusing), CE-SDS and fractionation. Get protein charge and size data on a variety of biotherapeutics—mAbs, ADCs, fusion proteins, vaccines, and more. Experience unparalleled ease-of-use, develop your methods in a day, and easily transfer them to QC. Whether you need a single solution or multiple capabilities, our Maurice platforms offer a range of products to meet your needs— icIEF, CE-SDS, or both, plus fractionation.
Meet the Maurice Family Scan the QR Code or Visit: bio-techne.com/maurice
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Discover More with the Maurice Family
Figure 04A. A comparison of size separation of NIST mAb obtained from the Maurice Turbo CE-SDS cartridge and the CE-SDS PLUS cartridge, run on the Maurice system. Both methods are comparable.
Figure 04B. Apparent pI comparison of 8 different AAV serotypes with Maurice icIEF using native fluorescence detection. Distinct charge profiles are obtained for each serotype, demonstrating how Maurice can be used to determine AAV identity.
CE-SDS with Maurice Obtain high throughput protein separation for analyzing size and purity of therapeutic proteins. Get rapid results in as little as 5.5 minutes and make informed decisions with high-resolution data.
D A T A N O T E S :
icIEF with Maurice Get high resolution, reproducible protein charge heterogeneity data in 10 minutes or sooner with whole-column imaging based on the iCE technology. Use real-time absorbance, or leverage native fluorescence for 4X sensitivity, using as little as 0.7 μg/mL of your precious samples.
F I G U R E // 04 A
CE-SDS Analysis of NIST mAb
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an ce
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PLUS 25 minutes
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NGHC
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Comparison of 8 Different AAV Serotypes with Maurice icIEF
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10,000 Type 1
Type 3
Type 2 1in5
Type 4
Full 5-2
Type 6
Type 8
Type 95,000 0
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5,000 0
10,000
5,000 0
10,000
5,000 0
10,000
5,000 0
10,000
5,000 0
10,000
5,000 0
10,000
pl 6.2 7.26.4 7.46.6 6.8 7
MauriceFlex Maurice Maurice C. Maurice S.
T E C H N I C A L S P E C S
icIEF Fractionation
icIEF (Both Cartridges)
CE-SDS (Both Cartridges)
Absorbance Detection
Fluorescence Detection
On-board Mixing
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Meet Our Instruments Scan the QR Code or Visit: bio-techne.com/instruments
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Scale When You Need It
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Stretch Your Samples Further
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