Antibodies Validated for Simple Western
Antibodies Validated for Simple Western
At Bio-Techne, we know efficiency, accuracy and reproducibility are of critical importance when designing and conducting high-throughput assays for drug discovery and development. Our Simple WesternTM platform is an automated, hands-free, capillary immunoassay device that combines protein separation with sensitive chemiluminescence and fluorescence immunodetection using conventional primary and conjugated secondary antibodies.
Experience the Next Level of Productivity and Precision on Your Path to Discovery
To Maximize Efficiency, Choose Bio-Techne Antibodies
Simple Western systems are open platforms, meaning the possibilities are almost endless when selecting your primary antibodies for assay development. R&D Systems’ legacy of precision and excellence in immunoassay development starts with our foundational expertise in antibody engineering and manufacturing. Combined with our carefully curated portfolio from Novus Biologicals, Bio-Techne holds the largest selection of Simple Western-validated antibodies on the market.
vs.Jess Western blot
3 hours 3 days
3 µL 10-20 µL
3-4 logs ~2.5 logs
Low pg Low ng
Quantitative
Time
Dynamic Range
Sample Volume
Sensitivity
Quantitative
Semi-Quantitative
Simple Western: Automated Quantitation from Cell and Tissue Lysate Samples in About 3 Hours
The Bio-Techne Advantage: Support at Every Step
When you choose Bio-Techne antibodies for your Simple Western experiments, our technical experts can fully support your entire workflow. Whether you have questions about your antibody selection or instrument operations, Bio-Techne’s technical service and customer support can help.
Scan to easily find Simple Western- validated antibodies at Bio-Techne.com.
Bio-Techne Simple Western-validated antibodies cover a range of research areas. Simple Western lane view showing detection of (A) Beta-III tubulin in rat cortical neurons, (B) Phospho-Akt (S473) in MCF-7 cells after treatment with IGF-1, and (C) LC3B in NIH3T3 cells after chloroquine treatment.
Bio-Techne® | R&D Systems™ Novus Biologicals™ Tocris Bioscience™ ProteinSimple™ ACD™ ExosomeDx™ Asuragen® For research use or manufacturing purposes only. Trademarks and registered trademarks are the property of their respective owners.
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Featured Pathways and Research Areas
Cell Signaling Pathways
Immuno-Oncology Extracellular Vesicles (EV)
GAPDH Carbonic Anhydrase IX/CA9
CD81
Tyrosine Hydroxylase Survivin CD63
HIF-1 alpha p53 TSG101
LC3B Phospho-p53 ALIX
c-Myc EGFR Flotillin-1
SOX17 PD-1 HSP90
Caspase 3 PD-L1/B7-H1 HSP60
Caspase 6 ErbB2/Her2 HSP70
Akt Phospho-ErbB2/Her2 Calnexin
Phospho-Akt TREM2
NFKB
ZAP70 Epitope Tag/ Reporter
Loading Controls
DNMT1 HA Tag GAPDH
p62 HIS Tag Beta-actin
ERK1/2 DYKDDDDK Tag Beta-lll Tubulin
Phosph-ERK1/2 GFP
For the full catalog of R&D Systems and Novus Biologicals Simple Western-validated antibodies, scan here:
Key Benefits of Bio-Techne Antibodies
Efficiency and Reliability: Streamline workflows with meticulously validated antibodies for rapid and reliable results.
Catalog Breadth and Depth: Bio-Techne antibodies optimize efficiency across diverse research areas.
Cutting-Edge Portfolio: Leveraging in-house instrumentation, our Simple Western-validated antibody portfolio is constantly growing to keep you at the forefront of progress.
Bio-Techne supports Simple Western-validated antibodies with in-house validation data, peer-reviewed citations, and customer reviews. Find recommended dilutions for your Simple Western experiments on our technical data sheets.
230
kDa
ß-lll Tubulin
180
116
66
40
12
kDa
LC3B
Chloroquine
NIH3T3
kDa +- +-IGF-I MCF-7
Rat Cortical Neuron
Phospho-Akt (S473)
230 180
116
66
40
12
230 180
116
66
40
12
230
kDa
ß-lll Tubulin
180
116
66
40
12
kDa
LC3B
Chloroquine
NIH3T3
kDa +- +-IGF-I MCF-7
Rat Cortical Neuron
Phospho-Akt (S473)
230 180
116
66
40
12
230 180
116
66
40
12
230
kDa
ß-lll Tubulin
180
116
66
40
12
kDa
LC3B
Chloroquine
NIH3T3
kDa +- +-IGF-I MCF-7
Rat Cortical Neuron
Phospho-Akt (S473)
230 180
116
66
40
12
230 180
116
66
40
12
A B
C