Meet Leo
The Simple Way to Maximize Throughput for Reproducible Protein Quantitation with High Specificity
Meet Leo
Leo System Simple Western
Precision & Th
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Reimagine Protein Analysis In today’s fast-paced research environment, traditional protein analysis methods fall short, slowing discovery with manual workflows, inconsistent data, and results you can’t fully trust. When precision matters and time is critical, you need more than just a workaround.
It’s time for a better way.
Introducing the Leo™ System, the next leap in Simple Western™ Technology—where high-throughput, hands- free automation meets quantitative accuracy. Say goodbye to compromise and hello to confidence in every result. Discover the simple way to do next-level protein quantitation.
Explore Leo with an immersive 3D experience and navigate Leo's features at your own pace in a virtual environment.
Take 3D Tour Scan the QR Code or Visit: embed.envoke-demos.com/bio-techne/ leo_platform/web.html
https://embed.envoke-demos.com/bio-techne/leo_platform/web.html?pdfSource=true_leo+system+brochure+summer+2025 https://embed.envoke-demos.com/bio-techne/leo_platform/web.html?pdfSource=true_leo+system+brochure+summer+2025
Meet Leo: The Ultimate Analytical Tool for Protein Quantitation The Leo System, powered by Simple Western Technology, is a high- throughput, fully automated capillary immunoassay platform designed to elevate your bioanalytics with unmatched speed, simplicity, and sensitivity. Built on proven Simple Western Technology, Leo offers reproducible protein detection and quantitation with exceptional efficiency. Process up to 100 capillaries per run, expanding your experimental capacity for quantitative assays and delivering reliable results in as little as 3 hours.
Automated Platform That is fast, hands-free, and reproducible
Accurate Protein quantitation with intra-assay, inter-run, and inter-instrument CVs <10%
Powerful Data Analysis Enables 21 CFR Part 11 Compliance
Upgradeable To fluorescence detection
High Precision With intra-assay peak area CV <15% and inter-run peak area CV <15%
3-4 log Dynamic range
Low Picogram Sensitivity Even from complex samples such as cell and tissue lysates
RePlex & Resample Detect up to 8 targets per sample
100 Capillaries Processed in a 3-hour run time
3 μL Sample Do more with your precious samples
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T H E L E O A D V A N T A G E
Request a Quote or Talk to a Specialist Today Scan the QR Code or Visit: bio-techne.com/leo
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https://www.bio-techne.com/leo?pdfSource=true_leo+system+brochure+summer+2025
Leo On the Inside
How Does Leo Work? Leo is built on proven and trusted Simple Western technology. It separates proteins by size using capillary electrophoresis. Separated proteins are immobilized on the capillary surface and can be identified using an immunoassay. Simply load your samples and reagents into the microplate, and Leo takes care of the rest. It precisely manages every step: protein separation by size, antibody additions, incubations, washes, and even detection. Return to thoroughly analyzed, quantified results in just 3 hours.
Leo holds up to 4 capillary cartridges, 4 peel and go pre-filled reagents and 1 sample plate with room for 96 samples and 4 molecular weight ladders per run. Supporting Leo is a complete set of reagents and consumables and Compass for Simple Western software. The capillary cartridges move back and forth between pre-filled reagent plates and the sample plate to load samples and reagents into the capillaries, perform protein separation, immobilization, and detection.
25-Capillary Cartridge Holders
Pre-filled Reagent Plates
Sample Plate4 4 1
0706
Load Sample
Load Matrix
Separate
Immobilize
Quantitate Signal6.
Immunoprobe
2.
1.
3.
4.
5.
HRP-Labeled 2˚ Ab
Target X Target Y
Target Y 1˚ Ab
Separation MatrixStacking Matrix
Electric Current
CE-ing is Believing Watch Video to See How Leo Works
Scan the QR Code or Visit: bio-techne.com/leo
Accommodates up to 96 samples + 4 Biotinylated Ladders
Samples
Antibody Diluent Biotinylated Ladder
Primary Antibody Streptavidin HRP Secondary Antibody Antibody Diluent Wash Buffer Luminol/Peroxide
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What Happens Inside a Capillary?
Leo Sample Plate
Built on Proven, Trusted Simple Western Technology
https://www.bio-techne.com/leo?pdfSource=true_leo+system+brochure+summer+2025
Discover More with Leo
Figure 01. A single 3-hour run, Leo has space for 4 cartridges to run 25 capillaries, translating to 4 MW ladders and up to 96 unique samples.
Figure 02. In this example, one Leo run was used to measure GFP transgene expression in HEK293 cells transduced with two lots of AAV8. An 8-point standard curve of recombinant GFP is run in triplicate on the 1st cartridge, and 16 samples are run in triplicate on cartridges 2–4. Furthermore, the RePlex feature was used for total protein normalization for more accurate quantification. Download the Application Note for more information.
Table 01. Cartridge correction further improves Intra-Assay Precision. FANCD2 average peak areas across four cartridges were measured on Leo. With the new cartridge correction feature in Compass for Simple Western software, potential variations between cartridges can be normalized. Cartridge correction improved the intra-assay CV to 5.0% compared to the intra-run CV of 6.8% without cartridge correction.
Figure 03. Three independent runs were preformed on the same Leo instrument analyzing FANCD2 peak area, using split samples and matched lots of reagents, pre-filled plates, and cartridges. Even without cartridge correction, the intra-run CVs are consistently low, ranging from 6.0% to 8.0%. With cartridge correction, the CVs are further reduced, ranging from 4.1% to 7.2%. This demonstrates the inherent precision of the Leo instrument and the effectiveness of the cartridge correction algorithm. The graph on the right displays the average peak area for each run after cartridge correction, along with the overall mean and lines representing plus/minus 10% and 20% deviation from the mean of all runs. Also, the inter-run precision of the data was calculated when using cartridge correction. The inter- run CV is notably low at 7.5%, which indicates that the Leo system delivers highly reproducible results across multiple runs, ensuring consistency and reliability in your experiments.
Process Up to 100 Capillaries in 3 Hours Leo allows you to process more samples in a day, with a capacity of up to 100 capillaries in a single 3-hour run.
Consistent Data Within & Between Runs Leo offers robust and consistent performance with low CVs across cartridges within and between runs. CVs that are less than 15% for well optimized assays underscores the high precision and reliability of Leo.
Large Experimental Size for Enhanced Quantitation Leo can accommodate up to 96 sample experiments in a single high-precision Leo run, providing enough room for large standard curves, replicates, calibrators, QC samples, and test samples.
F I G U R E // 0 1
3-Hour Leo Run Cartridge 1
96 Samples // 4 Ladders // 3-Hour Run
Cartridge 2 Cartridge 3 Cartridge 4
F I G U R E // 02
Large Experimental Size
AAV Lot 1 n = 4
AAV Lot 2 n = 4
Std. Curve n = 3
G FP
La dd
er
Cart. 1 Cart. 4 Intra-RunCart. 3Cart. 2
I NTR A-AS SAY P R EC I S I O N
3.8% 3.7% 6.8%5.3%5.8%% CV without Cartridge Correction
3.8% 3.7% 5.0%5.3%5.8%% CV with Cartridge Correction
Run 1
Run 2
Run 3
F I G U R E // 03
Leo Inter-Run Precision
300,000
400,000
500,000
600,000
700,000
800,000
+20%
-20%
+10%
Mean of Runs
-10%
Run 1 Run 2 Run 3 Average
With Cartridge Correction
FA N
C D
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A re
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Intra-Run %CV without Cartridge Correction
Intra-Run %CV with Cartridge Correction
8.0% 7.2%
6.0% 4.1%
6.8% 5.0%
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https://resources.bio-techne.com/bio-techne-assets/docs/literature/an-revolutionizing-gene-therapy-potency-assays-with-leo.pdf https://resources.bio-techne.com/bio-techne-assets/docs/literature/an-revolutionizing-gene-therapy-potency-assays-with-leo.pdf
Accurate Protein Quantitation You can confidently put your standard curve on any of the 4 Leo cartridges and use the other 3 to quantify your samples accurately. Recovery values within the standard 80–120% range expected for bioanalytical method validation can be obtained for well-optimized assays.
Excellent Inter-Instrument Precision Leo delivers highly consistent results across multiple instruments, ensuring data comparability and reliability regardless of the instrument used. This level of inter-instrument reproducibility is critical for studies involving multiple sites or laboratories. It allows for better comparison across different locations, ensuring instrument variability does not influence results. This is particularly important for large-scale collaborative studies, clinical trials, and other applications where data consistency is paramount.
Figure 04. Inter-instrument reproducibility supporting cross-site assay transferability. For 5 Leo instruments, the average peak area of FANCD2 was measured along with the overall mean and 10% and 20% deviations from the mean. The intra-run CVs were consistently low, ranging from 4.9% to 5.6%, and the inter-instrument precision, representing the variability between the five different Leo instruments, was 4.2%. The Leo instruments were run in parallel using split samples and the same lots of cartridges, reagents, and pre-filled reagent plates.
Figure 05. Spike and recovery study confirms excellent accuracy. Standard curves were defined for each cartridge independently within Compass or only for Cartridge 1. Then, the calculated concentrations were exported using either the individual standard curves from each cartridge (05A) or the standard curve from Cartridge 1 (05B), and the % recovery for standards and QC samples was determined by dividing Compass-calculated concentrations by known concentrations for the QC samples within each cartridge.
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Inter-Instrument Reproducibility
300,000
400,000
800,000
900,000
700,000
600,000
500,000
Leo 1 Leo 2 Leo 3 Leo 4 Leo 5
+10%
-10%
Run Mean
FA N
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2 A
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A re
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with Cartridge Correction
+20%
-20%
I NTE R- I N STR U M E NT P R EC I S I O N = 4.2% CV
Intra-Run %CVInstrument
5.4%
5.2%
5.0%
4.9%
5.6%
Leo 1
Leo 2
Leo 3
Leo 4
Leo 5
F I G U R E // 05A
Average % Recovery Using Intra-Cartridge Standard Curves
80%
90%
120%
110%
100%
0 50 100 150 200 250 300 Concentration (ng/mL)
Q C
S am
pl e
% R
ec ov
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A ve
ra ge
Cart. 1
Cart. 2
Cart. 3
Cart. 4
F I G U R E // 05 B
Average % Recovery Using Standard Curve from Cartridge 1
80%
90%
120%
110%
100%
0 50 100 150 200 250 300 Concentration (ng/mL)
Q C
S am
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% R
ec ov
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A ve
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Cart. 1
Cart. 2
Cart. 3
Cart. 4
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Figure 07. Leo can accurately quantify RNase A over a range spanning 3.5 logs and a range of 4 logs for DnaK. In addition to the broad dynamic range, the data points are tightly clustered across the three instruments, demonstrating excellent inter-instrument reproducibility and linearity within this range. The 3 Leo instruments were run in parallel using split samples and the same lots of cartridges, reagents and pre-filled reagent plates.
Figure 06. The limit of detection (LOD) was determined for two recombinant proteins, RNase A and DnaK, on 3 independent Leo systems. An 8-point serial dilution for each protein was prepared with 2.5-fold dilutions for RNase A and 3-fold dilutions for DnaK. These dilutions were spiked in HeLa lysate as background, and then the split samples were analyzed in parallel on the 3 Leo systems using lot-matched cartridges, reagents, and pre-filled reagent plates. All samples were run in triplicate within a cartridge, and resampling for the four cartridges provided 12 data points per sample for each Leo instrument.
Broad Dynamic Range A broad dynamic range is crucial for accurately quantifying proteins in applications where protein levels can vary significantly, such as biomarker discovery. Leo offers a broad 3-4 log dynamic range and excellent inter-instrument reproducibility and linearity within this range.
F I G U R E // 0 6A
RNase A F I G U R E // 0 6 B
DnaK
700
800
600
500
400
300
200
100
0
Leo 1 Leo 2
Assay Range 253-452 pg/mL
Leo 3
R N
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A L
O D
( pg
/m L)
Leo 1 Leo 2
Assay Range 322-449 pg/mL
Leo 3
D na
K L
O D
( pg
/m L)
700
800
600
500
400
300
200
100
0
F I G U R E // 07A
RNase A F I G U R E // 07 B
DnaK
10,000
100,000
1,000,000
10,000,000
1,000 0.1 1 10 100 1,000 10,000
Concentration (ng/mL)
3.5 logs of dynamic range
R N
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Leo 1
Leo 2
Leo 3
1,000
10,000
100,000
1,000,000
10,000,000
100 0.01 0.1 101 100 1,000 10,000
Concentration (ng/mL)
4 logs of dynamic range
D na
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A re
a
Leo 1
Leo 2
Leo 3
Picogram-Level Sensitivity High sensitivity is essential for many applications in biological research, and Leo enables the detection of proteins down to pg/mL levels, which can be critical to measure low abundance protein biomarkers or targets for therapeutic intervention
Straightforward Transfer and Comparable Performance Across Simple Western Platforms
Antibodies often have quite similar EC80 values on Jess and Leo, as well as similar dynamic range and LOD.
Figure 08. RNAse A dynamic range and limit of detection experiments across 2 Jess and 3 Leo instruments. Both Jess and Leo produced 3.5 log dynamic ranges for RNAse A.
10,000
100,000
1,000,000
10,000,000
100,000,000
1,000 1 10 100 1,000 10,000
Concentration (ng/mL)
3.5 logs of dynamic range across 3 Leo and 2 Jess
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Leo 1 Jess (n=2)
Leo 2
Leo 3
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Figure 09. Leo combines RePlex with resampling, offering a powerful multiplex option to get the most data from your precious samples. RePlex enables serial probing of the same capillary with 2 immunoassays, or an immunoassay followed by total protein analysis. Total protein detection happens in the same capillary on the same sample and offers more reliable protein quantification since it normalizes out experimental variation in protein loading
Get More Data from Your Precious Samples While Saving on Reagents with Leo
Leo combines RePlex with resampling, offering a powerful multiplex option to assess up to 8 target proteins from just 3 µL of sample. This ability to perform multiple analyses from the same sample reduces sample consumption and minimizes the need for repetitive sample preparation. By directing all 4 cartridges to the same row of primary and secondary antibodies, you can save up to 50% on your primary and secondary antibodies.
Resampling 1° & 2° Antibody Wells • Save up to 50% on 1° & 2° Antibodies
D A T A N O T E S
RePlex + Resampling Sample Wells • Enables analysis of up to 8 target proteins
on 25 samples for 100 capillary experiment.
OR
• Run up to 4 immunoassays followed by a total protein assay
• Eliminate concerns with antibody cross-reactivity when multiplexing
Load Matrix & Sample
Separate & Immobilze
RePlex (Remove Antibodies from 1st Immunoprobe)
Detect & Quantitate Target 2
7.
Antibody 2 Immunoprobe (or Total Protein Assay)
1.
2.
5.
6.
Detect & Quantitate Target 1
4.
HRP-Labeled 2˚ AbTarget Y 1˚ Ab
Antibody 1 Immunoprobe
3.
HRP-Labeled 2˚ AbTarget Y 1˚ Ab
Electric Current
Target X Target Y
RePlex Enables Serial Probing: 2 immunoassays OR 1 immunoassay + total protein normalization
Resampling: Up to 4 cartridges can be directed to the same row of wells on sample plate
1 Sample Row // Eight 1° Ab Rows // Two 2° Ab Rows 4 Sample Rows // One 1° Ab Row // One 2° Ab Row
+ OR
F I G U R E // 0 9
Leo Combines RePlex & Resampling
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Powerful Software with Advanced Analysis Features & Support for 21 CFR Part 11 Compliance Leo comes with Compass Software for Simple Western that streamlines data analysis with an intuitive interface and robust tools, including built-in standard curves and annotation features. It also enables 21 CFR Part 11 compliance to meet regulatory requirements.
Advanced Standard Curve Tools • Compass Software has in-software tools to build a
standard curve and generate reported concentrations of unknowns.
• Compass for Simple Western v7 supports the following curve fits: Linear, 4PL, Log-Log.
Sample Group Analytics • Group samples together by attributes defined in
the Assay Layout, such as Sample Name, Attribute, or more.
• Compare your replicates and get variability data quickly and easily with sample grouping.
Leo Assay Setup and Analysis • Compass for SW v7 lets you set up assays for Leo using
25–100 capillaries and 1–4 cartridges.
• Normalizes cartridge-to-cartridge variability using a reference control across each cartridge, allowing for quantitative comparisons between data from one cartridge and another.
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Unleashing Analytical Applications with Leo Leo is used in a wide variety of applications where traditional methods struggle to deliver with enough speed and quantitation. These include high- throughput targeted protein degradation, relative potency assays for QC and lot release for cell and gene therapy products and measuring key biomarkers in clinical trials. Researchers also use Simple Western for diverse applications, including vaccine development, bioprocess impurity testing, pharmacokinetics/ pharmacodynamics, and measuring target expression in different tissues.
Figure 10. Simple Western Platforms have been utilized in various research areas, from cancer biology and neuroscience to drug discovery and development. *Source: Effect of NFX-179 MEK inhibitor on cutaneous neurofibromas in persons with neurofibromatosis type 1. Sci Adv. 2024 May 3;10(18):eadk4946.
F I G U R E // 1 0
Simple Western Research Areas F I G U R E // 1 0
Simple Western Research Areas
Relative Potency Assays for QC Lot Release
Normalized Values 2
4
6
8
3 4 5 6 7 AAV8 MOI (log vg/cell)
G FP
N or
m al
iz ed
A re
a (lo
g)
Lot 1 (Control)
Lot 2 (Control) p ER
K: to
ta l E
R K
(% )
0
5
10
15
20
25
30 -10.5%
P=0.036
-26.2%
-46.9%
35
40
Reduction vs.
Vehicle
Day 28 Vehicle
30.4 27.2 22.4 16.1
Gel 0.05% Gel 0.15% Gel 0.50%
Clinical Biomarkers* P=0.0001
log[drug] (M)
% D
M SO
C on
tr ol
125
100
75
50
25
0 -10 -9 -8 -7 -6 -5 -4
High Throughput Targeted Protein Degradation Curves
116 440 MW (kDa)
0
2
4
6
8
10
12
C he
m i.
(R LU
x 1
0, 0
0 0)
Full 75/25 (Full/Empty) 50/50 (Full/Empty) 25/75 (Full/Empty) Empty
Cell & Gene Therapies
0 20 40 60 80 100 0
2
4
6
8
10 AAV5 Empty/Full Standard Curve
AAV5 E/F Standards (% Full)
D N
A P
ea k
A re
a (x
1 0
6 )
R2 = 0.9925
Batch 1 = 684.0 ng/mL BSA
Batch 2 = 3,781.3 ng/mL BSA
Batch 3 = 746.6 ng/mL BSA
Batch 4 = 1,663.7 ng/mL BSA
MW (kDa)
5,000
5,000
5,000
5,000
0
0
0
0C he
m ilu
m in
es ce
nc e
30 100 200
Vaccine Development
Brain Tissue (Novus Biologicals) Brain Tissue (ProSci) Endothelial Cells Cervical Cancer Cells
12 40 66 116 180 230 MW (kDa)
0
2
4
6
8
10
12
14
16
18
C he
m ilu
m in
es ce
nc e
(R LU
x 1,
00 0)
Tissue Biodistribution
12 40 66 116 180230 MW (kDa)
0
1
2
3
4
5
6
C he
m ilu
m in
es ce
nc e
(R LU
x 1
00 ,0
00 ) Bioprocess Impurity Detection
High QC 400 ng/mL
Low QC 10 ng/mL
10-1 100 101 102 103 104 103 104 105 106 107 108
NucA Protein (ng/mL)
A re
a (M
ea n)
Low QC
High QC NucA Standard Curve Main Peak Biosimilar 1
Innovator
Biosimilar 2
0
5
10
15
20
25
30
35
40
Av er
ag e
Pe ak
A re
a (x
10 ,0
00 )
Time at 37 °C F roz
en
Ct rl.
0 h 1 h 4 h 8 h 12 h
24 h
48 h
72 h
96 h
12 0 h
14 4 h
16 8 h
19 2 h
2 w ks
3 w ks
4 w ks
9 w ks
PK/PD
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Additional Modules
Sample Plates
Detection Modules (600 capillaries)
Detection Modules (200 capillaries)
Separation Modules (200 capillaries)
Pre-filled Plates & Reagents Kit (200 capillaries)
Separation Modules (600 capillaries)
Pre-filled Plates & Reagents Kit (600 capillaries)
AS SAYS & C O N S U M A B LES
RePlex (RP-001)
Leo Sample Plate Kit, 2 Plates (SWSP-001)
Anti-Rabbit (SWDM-021)
Anti-Rabbit (DM-001)
12-230 kDa (SWSM-W014)
12-230 kDa (SWPR-W014)
12-230 kDa (SWSM-W015)
12-230 kDa (SWPR-W015)
Total Protein, 600 cap. (SWDM-TP21)
Leo Sample Plate Kit, 6 Plates (SWSP-002)
Anti-Mouse (SWDM-022)
Anti-Mouse (DM-002)
66-440 kDa (SWSM-W016)
66-440 kDa (SWPR-W016)
66-440 kDa (SWSM-W017)
66-440 kDa (SWPR-W017)
Total Protein, 200 cap. (DM-TP01)
Anti-Goat (SWDM-026)
Anti-Goat (DM-006)
2-40 kDa (SWSM-W018)
2-40 kDa (SWPR-W018)
2-40 kDa (SWSM-W019)
2-40 kDa (SWPR-W019)
Anti-Human IgG (SWDM-025)
Anti-Human IgG (DM-005)
No Secondary (SWDM-023)
No Secondary (DM-003)
Biotin (SWDM-024)
Biotin (DM-004)
Leo Consumables
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https://www.bio-techne.com/p/simple-western/replex-module_rp-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/replex-module_rp-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-sample-plate-kit-2-plates_swsp-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-sample-plate-kit-2-plates_swsp-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-sample-plate-kit-2-plates_swsp-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-anti-rabbit-detection-module-chemiluminescence-for-24-x-25-samples_swdm-021?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-anti-rabbit-detection-module-chemiluminescence-for-24-x-25-samples_swdm-021?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/anti-rabbit-detection-module_dm-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/anti-rabbit-detection-module_dm-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-12-230-kda-separation-module-for-8-x-25-samples_swsm-w014?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-12-230-kda-pre-filled-plates-reagents-kit-8-x-25-caps_swpr-w014?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-12-230-kda-separation-module-for-8-x-25-samples_swsm-w014?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-12-230-kda-pre-filled-plates-reagents-kit-8-x-25-caps_swpr-w014?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-total-protein-detection-module-chemiluminescence-for-24-x-25-samples_swdm-tp21?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-total-protein-detection-module-chemiluminescence-for-24-x-25-samples_swdm-tp21?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-total-protein-detection-module-chemiluminescence-for-24-x-25-samples_swdm-tp21?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-sample-plate-kit-2-plates_swsp-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-sample-plate-kit-2-plates_swsp-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-sample-plate-kit-2-plates_swsp-001?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-anti-mouse-detection-module-chemiluminescence-for-24-x-25-samples_swdm-022?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-anti-mouse-detection-module-chemiluminescence-for-24-x-25-samples_swdm-022?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/anti-mouse-detection-module_dm-002?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/anti-mouse-detection-module_dm-002?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/simple-western-66-440-kda-separation-module-for-8-x-25-samples_swsm-w016?pdfSource=true_leo+system+brochure+summer+2025 https://www.bio-techne.com/p/simple-western/leo-66-440-kda-pre-filled-plates-reagents-kit-24-x-25-caps_swpr-w016?pdfSource=true_leo+system+brochure+summer+2025 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Leo Total Protein Assay
Leo Chemiluminescence Immunoassay
R E P R ES E NTATI V E P E R FO R M A N C E DATA*
S P EC I FI CATI O N S
<15%**<15%**
<3 hours, RePlex: 5 hours
Intra-assay Peak Area CV (%)
Runtime
<15%***<15%*** 100
Inter-run Peak Area CV (%) Max Capillaries Per Run
N/A<10%****96 Intra-assay Quantitation CV (%)Samples Per Run
N/A<10%**** 3 µL
Inter-run Quantitation CV (%) Volume Required
N/A<10%****
No
Inter-instrument Quantitation CV (%)
Sample Cooling
2–3 logs3–4 logs
Chemiluminescence (upgradeable to Fluorescence)
<10%
72 x 74 x 74 cm / 28.3 x 29.1 x 29.1 inch
Dynamic Range
Detection
Sizing CV (%)
Dimensions (closed) (H x W x D)
ngLow pg
Yes
± 10% for MW >20 kDa ± 15-20% for MW <20 kDa
72 x 74 x 122 cm / 28.3 x 29.1 x 48 inch
18–23 ℃
Sensitivity
RePlex
Resolution (± Difference in MW)
Dimensions (open) (H x W x D)
Operating Temperature
0.6–1.2 µg†
Molecular weight (MW) ladder ranges from 2–440 kDa
350 lbs
0.3–1.2 µg†
US/CAN 120 V AC, 60 Hz, 5.0 amps China 220 V AC, 50 Hz, 2.7 amps Europe 230 V AC, 50 Hz, 2.6 amps Japan 100 V AC, 50/60 Hz, 6.0 amps
20–60%
Size Range
Weight
Sample Required
Power
Operating Relative Humidity (non-condensing)
*Representative performance data for well-characterized assays using split samples, with the same lots of reagents, plates, and cartridges for each experiment. **Based on a model assay system with a signal-to-noise ratio >250, n=96, and cartridge correction. ***Based on model assay system with a signal-to-noise ratio >250, n=3, and cartridge correction. ****%CV of calculated concentration generated using a standard curve for all samples above LLOQ using cartridge correction. †Sample volume required is 3 µL/well
Leo Instrument Specifications
B IO
-T E
C H
N E
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E E
T L
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2524
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Simple Western Proven, Trusted Technology
3,000+ Publications on PubMed
Thousands Of Instruments Installed
6,500+ Validated Antibodies
Leo The simple way to maximize throughput for quantitative western results.
Abby The simple way to faster publication-ready western results.
Jess The simple way to experience powerful multiplex western results.
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