RNA ISH ASSAY TO DETECT ASO, miRNA, OR siRNA

THE miRNAscope™ ASSAY

WITH SPATIAL AND MORPHOLOGICAL CONTEXT AT SINGLE-CELL RESOLUTION

VISUALIZE SMALL RNAs WITH MORPHOLOGICAL CONTEXT AND SPACIAL RESOLUTION RNAscope™ technology is an advanced in situ hybridization assay that allows for the visualization of single-molecule gene expression directly in intact tissues with single cell resolution. The assay represents a major advancement in RNA ISH approaches with its proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridization.

Detection of small RNAs requires a robust, highly specific, and highly sensitive assay with minimal time, ease of effort, and ease of data interpretation. While microarrays and PCR both provide useful molecular profiles of diseases, important clinically relevant cell and tissue context information is lost along with the spatial variation of gene expression patterns. The miRNAscope Assay is an advanced in situ hybridization assay leveraging the RNAscope core technology that allows for the visualization of small RNA expression in intact tissues or cultured cells with single-cell resolution.

UNLOCK THE POTENTIAL TO DETECT SMALL RNAs WITH THE NEW miRNAscope ASSAY

• Detect and identify cellular subtypes • Visualize gene regulation with morphological context • Validate miRNA biomarkers in intact tissues • Assess small RNA therapeutic delivery mechanism • Evaluate biodistribution and efficacy of therapy • Add a visual dimension to heterogeneous tissue biology and analysis

” “We had a great experience using miRNAscope in our compound screening efforts. The technique is incredibly specific and the results are very easy to

understand. Also, working with ACD’s team has been extremely beneficial. They communicated with us during the process to ensure we can get the answers to our questions. They generated impeccable tissue stainings!

- A Leading BioPharma Company in NY, USA

THE miRNAscope ASSAY DEMONSTRATES EXCEPTIONAL PERFORMANCE FOR THE DETECTION OF: • Antisense oligonucleotides (ASOs) • Small interfering RNAs (siRNAs) • microRNAs (miRNAs) • Small RNA sequences that are 17-50nt in length

miRNAscope ASSAY BENFITS

VISUALIZE AT THE SINGLE-CELL LEVEL WITH UNMATCHED RESOLUTION

ASO Example detection of ASO target (yellow arrows) in the treated mouse quad muscle tissue samples and not in the vehicle samples indicates success- ful delivery of ASO therapy.

miRNA ISH-IHC Example of miRNAscope ISH-IHC staining for miR-223-3p (yellow arrows) / CD3 (black arrows) in human cervical cancer. T cell infiltration with- in cervical tumor visualized using CD3-IHC in combination with myeloid-specific miRNAscope miR-223-3p probe.

siRNA Example of specific detection of the siRNA#1 probe (yellow arrows) in siRNA#1 treated mouse liver tissue samples only and not in the negative saline nor siRNA#2 treated samples indicates successful deliv- ery of siRNA therapy and specificity of the miRNA- scope Assay. Biodistribution of siRNA target is also observed in the siRNA#1 treated samples.

Note: Above tissue slides were counterstained with hematoxylin to provide blue staining of cells.

VEHICLE (neg. control)

SALINE (neg. control) siRNA #1 delivered siRNA #2 delivered

(neg. control)

TREATED

SPEED • Simple and fast workflow with both manual and automated formats to fit your need

• Rapid probe design to target almost any small markers (17-50nt) in any species

EASE OF USE • An out-of-the-box solution

• Hands on support and guidance from experienced in-house scientists

SPECIFICITY • Deeper evaluation of target in tissue context

• High signal-to-noise ratio for ease of data interpretation

SENSITIVITY • Spatial context with subcellular resolution

• Compatible with multiple sample types

PRODUCT OFFERINGS

LATEST ADDITION

OTHER OFFERINGS

miRNAscope™ Assay

Chromogenic Singleplex Brown

Features

Chromogenic Singleplex Red

Chromogenic Singleplex Red

Manual Assays

Automated on Leica BOND RX System

ISH-IHC Compatibility

Chromogenic Duplex

Fluorescent Multiplex

HiPlex

Manual assays

Automated on Ventana DISCOVERY ULTRA System

RNAscope®

Assay

BaseScope™

Assay

Pharma Assay Services

Manual assays

Automated on Ventana DISCOVERY ULTRA System

Automated on Leica BOND RX System

Manual assays

Automated on Ventana DISCOVERY ULTRA System

Automated on Leica BOND RX System

Automated on Leica BOND RX System

CONTACT ACD Information Email: info.acd@bio-techne.com Orders Email: order.acd@bio-techne.com Support Email: support.acd@bio-techne.com Get started today: www.acdbio.com/go 7707 Gateway Boulevard, Newark, CA 94560 1.510.576.8800 (Main) | 1.877.576.3636 (Toll Free)

bio-techne.com

Global info@bio-techne.com bio-techne.com/find-us/distributors TEL +1 612 379 2956 North America TEL 800 343 7475 Europe | Middle East | Africa TEL +44 (0)1235 529449 China info.cn@bio-techne.com TEL +86 (21) 52380373 For Research Use Only. Not for diagnostic use. RNAscope, BaseScope, and miRNAscope are trademarks of Advanced Cell Diagnostics, Inc. in the United States or other countries. All rights reserved. 2020 Advanced Cell Diagnostics, Inc. Doc# MK 51-146 RevA/Effective Date 7/20/2020

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