CHOOSING THE RIGHT ELISA

SELECTING THE ELISA FORMAT THAT BEST MEETS YOUR RESEARCH NEEDS From our renowned Quantikine™ ELISA kits to our fully automated Simple Plex™ assays, Bio-Techne offers several different ELISA formats to meet a broad range of analysis and biodiscovery needs.

While the results you can achieve from various types of ELISAs may be similar, the preparation steps, testing times, and sample volume requirements may differ entirely.

How do you select the type of ELISA that will best meet your speed, efficiency, and accuracy requirements? The determining factors often center around core issues like required sensitivity, speed of results, and economical considerations.

Use the side-by-side comparison table below to determine which ELISA format would be the best fit for your research needs.

ICON DESCRIPTION FORMAT NUMBER OF

ANALYTES BENEFIT

SAMPLE VOLUME

ASSAY TIME INSTRUMENT

Quantikine ELISA

96-well plate 1 Most published/

low CVs 10-200 µL 3 - 5 hours Plate Reader

Quantikine HS ELISA

96-well plate 1 Highest

sensitivity 10-200 µL 4 - 4.5 hours Plate Reader

Quantikine QuicKit ELISA

96-well plate 1 Fastest

Plate-based ELISA

50 µL 90 minutes Plate Reader

QuantiGlo ELISA

96-well plate 1 Broad dynamic

range 10-200 µL 4 - 4.5 hours Luminometer

DuoSet ELISA Flexible 1 Economical/ large menu

100 µL 20 hours Plate Reader

Simple Plex Assay

Cartridge Up to 8 Hands-free/

sensitive 2.5-25 µL 90 minutes Ella

WHICH ELISA IS RIGHT FOR YOU

Learn more | Read the ELISA Guide

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CHOOSE YOUR ELISA WITH CONFIDENCE Bio-Techne develops its ELISAs from raw materials that we manufacture in-house, giving us unparalleled control over critical elements that affect your results. Bottom line: you can count our ELISAs to deliver accurate, high-quality data consistently over time.

Bio-Techne antibodies are highly specific and carefully tested to ensure reproducibility and tested for suitability on every application we develop. This “quality-at-the-core” framework means you get accurate, reliable results—the first time and every time.

QUANTIKINE COLORIMETRIC SANDWICH ELISA KITS

Quantikine ELISA Kits are complete, ready-to-use kits that represent the gold standard in single analyte detection. Kits are available for measuring a wide range of molecules including cytokines, chemokines, growth factors, proteases, and more.

QUANTIKINETM HS (HIGH SENSITIVITY) COLORIMETRIC SANDWICH ELISA KITS Quantikine HS ELISA Kits are complete assays generally used when very low levels of the target protein are expected. These kits utilize distinct protocols to achieve impressive levels of analyte detection. Two different amplification systems are available.

QUANTIGLOTM CHEMILUMINESCENT SANDWICH ELISA KITS QuantiGlo ELISA Kits use a chemiluminescent substrate for analyte detection and require a luminometer for output reading. These kits have a broad dynamic range in comparison to standard colorimetric immunoassays.

Learn more | Read the ELISA Guide

QUANTIKINE QUICKIT COLORIMETRIC SANDWICH ELISA KITS QuicKit ELISA Kits allow for rapid quantification of target protein in only 90 minutes. With a shortened protocol and only one wash step, these ready-to-use kits allow you to accomplish more in your day while getting the same quality as a Quantikine ELISA kit.

DUOSET DEVELOPMENT SYSTEMS DuoSet ELISA Development Systems contain the basic components required to develop a sandwich immunoassay for accurately measuring analytes in biological fluids. DuoSets offer an economical alternative to buying separate antibodies and are adaptable across multiple platforms, including bead- based and 384 well plates.

SIMPLE PLEX ASSAYS Simple Plex Assays on the automated Ella platform represent the next-generation ELISA. In just 90 minutes you get highly reproducible validated assay data with no manual steps. The entire assay happens within the microfluidic cartridge, which allow Simple Plex assays to achieve a high level of sensitivity, precision, and throughput.

ASSAY CUSTOM SERVICES Looking for an analyte or ELISA kit that is not available on our product listing? Bio-Techne will work closely with you to produce assays that are tailored to your needs. This may include performance optimization of analytes from our menu of over 1000 analytes, or the creation of an assay for a biomarker that we don’t currently offer in any product categories. Explore options.

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