Cultrex™ Extracellular Matrix And Proteins

• Different formulations to meet evolving research needs and to match your cell’s microenvironment.

• MAP tested as raw materials and the final product is tested by PCR for mycoplasma and 30 other pathogens and viruses.

CultrexTM Basement Membrane Extract (BME) • Developed and manufactured to the highest standards in

the industry.

• The lowest level of endotoxins by competitive comparison.

• Lot-to-lot product consistency and comprehensive performance testing.

CultrexTM BME Selection Chart

NAME BUFFER CONCENTRATION* RECOMMENDED APPLICATIONS

UltiMatrix™ DMEM 10-12 mg/mL Stem Cell, Organoid, 2D and 3D Culture, Xenograft/Tumorgraft

Cultrex BME, Type 1 DMEM 8-12 mg/mL General 2-D Culture, 3-D Culture, Attachment, Invasion, etc.

Cultrex Stem Cell Qualified BME DMEM 8-12 mg/mL Stem Cell Culture

Cultrex 3-D Culture Matrix™ BME DMEM 8-12 mg/mL 3-D Culture

Cultrex Organoid Qualified BME, Type 2 DMEM 8-12 mg/mL Organoid Culture

Cultrex BME, Type 3 RPMI 1640 8-12 mg/mL Xenograft/Tumorgraft

Cultrex BME, Type R1 DMEM 8-12 mg/mL Organoid Culture

*measured by the Lowry assay

Cultrex BME Specifications

Name Endotoxin Sterility Mycoplasma PathClear Tube Stem Cell 3D Culture Organoid Tumor Growth High Entactin

UltiMatrix ✓ ✓ ✓ ✓ ✓ ✓ ✓

Type 1 ✓ ✓ ✓ ✓ ✓

Stem Cell Qualified ✓ ✓ ✓ ✓ ✓ ✓

3-D Culture Matrix ✓ ✓ ✓ ✓ ✓ ✓

Type 2 ✓ ✓ ✓ ✓ ✓ ✓

Type 3 ✓ ✓ ✓ ✓ ✓

Type R1 ✓ ✓ ✓ ✓ ✓ ✓ ✓

Assay Specification

Endotoxin Endotoxin ≤ 8 EU/mL by LAL assay.

Sterility Tested negative for the presence of bacteria and fungi.

Mycoplasma No mycoplasma contamination detected by PCR.

Pathclear Tested negative by PCR for 17 bacterial and viral strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.

Tube Promotes differentiation of human umbilical vein endothelial cells (HUVEC) into capillary-like structures.

Stem Cell Attachment and maintenance of human iPSCs in a pluripotent state in a feeder-free culture.

3-D Culture Promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) and human prostate (PC-3) into acinar structures.

Organoid Promotes growth and expansion of mouse small intestine organoid progenitor cells.

Tumor Growth Supports proliferation and growth of breast cancer cells (MCF7) embedded in the matrix for minimum of 8 days.

Entactin Entactin content ≥ 8% by SDS-PAGE.

Cultrex purified extracellular matrix (ECM) proteins provide a defined substrate for cell adhesion in culture. To accommodate a variety of different cell models, a full range of ECM component proteins have been developed

Cultrex Purified Extracellular Matrix Proteins including Laminin I, Collagen I, Collagen IV, Fibronectin, and Vitronectin. These include purified proteins that have been qualified for 3-D or stem cell cultures, as well as poly-L- lysine, poly-D-lysine and poly-L-ornithine.

Name Size Catalog #

Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract

1 mL BME001-01

5 mL BME001-05

2 x 5 mL BME001-010

Cultrex Basement Membrane Extract

1 mL 3432-001-01

5 mL 3432-005-01

2 × 5 mL 3432-010-01

Cultrex Reduced Growth Factor Basement Membrane Extract

1 mL 3433-001-01

5 mL 3433-005-01

2 × 5 mL 3433-010-01

Cultrex Stem Cell Qualified, Reduced Growth Factor Basement Membrane Extract

1 mL 3434-001-02

5 mL 3434-005-02

2 mL 3434-010-02

Cultrex 3-D Culture Matrix™, Basement Membrane Extract, Reduced Growth Factor

1 mL 3445-001-01

5 mL 3445-005-01

2 × 5 mL 3445-010-01

Cultrex Basement Membrane Extract, Type 2

1 mL 3532-001-02

5 mL 3532-005-02

2 × 5 mL 3532-010-02

Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2

1 mL 3533-001-02

5 mL 3533-005-02

2 × 5 mL 3533-010-02

Cultrex Basement Membrane Extract, Type 3

1 mL 3633-001-02

5 mL 3633-005-02

2 × 5 mL 3633-010-02

Name Size Catalog #

Cultrex Reduced Growth Factor Basement Membrane Extract, Type R1

1 mL 3433-001-R1

5 mL 3433-005-R1

2 × 5 mL 3433-010-R1

Cultrex Mouse Laminin I, PathClear 1 mg 3400-010-02

Cultrex Stem Cell Qualified Mouse Laminin 1 mg 3400-010-03

Cultrex Mouse Collagen IV 1 mg 3410-010-01

Cultrex Human Fibronectin, PathClear 1 mg 3420-001-01

Cultrex Stem Cell Qualified Human Fibronectin, PathClear 1 mg 3420-001-03

Cultrex Human Vitronectin, PathClear 50 µg 3421-001-01

Cultrex Stem Cell Qualified Human Vitronectin, PathClear 200 µg 3421-001-03

Cultrex Poly-L-Ornithine 100 mL 3436-100-01

Cultrex Poly-D-Lysine 100 mL 3439-100-01

Cultrex Poly-L-Lysine 100 mL 3438-100-01

Cultrex Rat Collagen I (5 mg/mL) 1 mL 3440-005-01

20 mL 3440-100-01

Cultrex Bovine Collagen I (5 mg/mL) 1 mL 3442-005-01

10 mL 3442-050-01

Cultrex Rat Collagen I, Lower Viscosity (3 mg/mL)

35 mL 3443-100-01

1 mL 3443-003-01

Cultrex 3-D Culture Matrix Laminin I 30 mg 3446-005-01

Cultrex 3-D Culture Matrix Rat Collagen I 20 mL 3447-020-01

Learn more | bio-techne.com

STRY0250084_CGT_FL_Cultrex Update_FR

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