Proteins for Stem Cell Culture Brochure.PDF
Proteins for Stem Cell Culture
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Key Benefits of Using R&D Systems Proteins for Stem Cell Culture
• High Levels of Biological Activity: The biological activity of every protein we offer is tested in an appropriate bioassay to confirm that it meets our strict QC activity parameters.
• Lot-to-Lot Consistency: Minimal lot-to-lot variability is ensured by testing each new lot side-by-side with previous lots and with a master lot, so you don’t have to worry whether results will be reproducible over time.
• High Purity and Low Endotoxin Levels: Our proteins are typically over 95% pure and have a guaranteed industry- leading endotoxin level of <0.1 EU/ug by the LAL method.
• Highly Cited: R&D Systems proteins commonly used for culturing stem cells and organoids are highly cited in the scientific literature.
• Seamless Transition from Preclinical Research to Clinical Manufacturing: R&D Systems Animal-free Preclinical and Animal-free GMP proteins frequently originate from the same clone, sequence, and expression system to make the transition from preclinical research into clinical manufacturing as seamless as possible.
• Supply Chain Reliability: Our team has the experience and the capacity to ensure that we can provide you with
a stable supply of the proteins needed for your research now and into the future.
• Bulk Proteins at Discounted Prices: We have the ability to scale up the production of any protein and we offer economical pricing on bulk orders.
• Custom Protein Capabilities: For specialized protein requests, you can always contact our Custom Protein Services team. Whether you are looking for a different formulation of a protein, incorporation of a non-standard label, or custom bottling, we have the capabilities and the team to develop the protein that you need.
• Comprehensive Portfolio of Reagents for Your Entire Stem Cell and Organoid Culture Workflows: Along with our proteins, we also offer a wide range of other products for stem cell and organoid culture and characterization, including Cultrex™ Basement Membrane Extracts, media and media supplements, Tocris™ small molecules, antibodies, ELISA Kits, RNAscope™ ISH assays, and analytical instruments to automate different steps of your workflow.
OPTIMIZE YOUR STEM CELL CULTURES WITH R&D SYSTEMS™ CYTOKINES AND GROWTH FACTORS. OUR PROTEINS PROVIDE HIGH LEVELS OF BIOACTIVITY AND LOT-TO-LOT CONSISTENCY SO YOU CAN HAVE CONFIDENCE IN THEIR ABILITY TO PROMOTE ROBUST STEM CELL
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EXPANSION AND DIFFERENTIATION WITH MINIMAL VARIABILITY BETWEEN CULTURES. FROM BASIC RESEARCH TO PRECLINICAL APPLICATIONS, WE OFFER RESEARCH-GRADE, ANIMAL-FREE, AND GMP PROTEINS TO MEET ALL YOUR EXPERIMENTAL NEEDS.
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Table 1.
Proteins for Stem Cell Culture
Molecule Species Source Catalog # Stem Cell Type
Activin A* Human/Mouse CHO 338-AC ESC, iPSC,
BDNF Human CHO 11166-BD NSC
BMP-2* Human/Mouse E. coli 355-BEC
ESC, iPSC, MSC, NSC CHO 355-BM
BMP-4* Human E. coli 314-BPE
ESC, iPSC, HSC, MSC, NSC Human NS0 314-BP
BMP-6 Human NS0 507-BP MSC
BMP-7 Human CHO 354-BP ESC, iPSC
CNTF Human E. coli 257-NT NSC
Dkk-1 Human Sf21 (baculovirus) 5439-DK ESC, iPSC
EGF* Human E. coli 236-EG ESC, iPSC, MSC, NSC
Erythropoietin Human CHO 287-TC HSC
FGF basic/FGF2/bFGF* Human E. coli 3718-FB
ESC, iPSC, MSC, NSC Human E. coli 4114-TC
FGF-3 Human E. coli 1206-F3 ESC, iPSC
FGF-4 Human E. coli 7460-F4 ESC, iPSC, MSC, NSC
FGF-7/KGF* Human E. coli 251-KG ESC, iPSC
FGF-8 Human E. coli 4745-F8
NSC Human/Mouse E. coli 423-F8
FGF-9 Human Sf21 (baculovirus) 273-F9 ESC, iPSC
Human HEK293 11233-F9 ESC, iPSC
FGF-10 Human E. coli 345-FG ESC, iPSC, NSC
FGF-17 Human E. coli 319-FG ESC, iPSC
FGF-18 Human E. coli 8988-F18 ESC, iPSC
FGF-19 Human E. coli 969-FG ESC, iPSC
* GMP-grade proteins are available for these molecules.
https://www.bio-techne.com/t/activin-a/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/bdnf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/bmp-2/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/bmp-4/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/bmp-6/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/bmp-7/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/cntf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/dkk-1/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/egf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/erythropoietin-epo/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-basic-fgf2-bfgf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-3/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-4/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/kgf-fgf-7/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-8/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-9/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-10/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-17/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-18/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/fgf-19/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture
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* GMP-grade proteins are available for these molecules.
Molecule Species Source Catalog # Stem Cell Type
Flt-3 Ligand/FLT3L*
Human HEK293 308-FKHB
ESC, iPSC, HSC Human HEK293 11309-FKH
Human NS0 308-FKN
Human E. coli 308-FKE
G-CSF Human E. coli 214-CS HSC
GDF-5/BMP-14 Human E. coli 8340-G5 MSC
GDNF Human NS0 212-GD NSC
GM-CSF* Human E. coli 215-GM
HSC Human CHO 7954-GM
HB-EGF Human Sf21 (baculovirus) 259-HE MSC
HGF* Human NS0 294-HGN ESC, iPSC
IFN-g* Human E. coli 285-IF
HSC Human HEK293 10067-IF
IGF-I/IGF-1* Human E. coli 291-G1 ESC, iPSC, MSC, NSC
IL-2* Human E. coli BT-002
HSC Human CHO 10453-IL
IL-3* Human E. coli 203-IL ESC, iPSC, HSC
IL-4* Human E. coli BT-004
HSC Human CHO 6507-IL
IL-6* Human E. coli 206-IL
ESC, iPSC, HSC, MSC Human HEK293 7270-IL
IL-7* Human E. coli BT-007
HSC Human HEK293 11089-IL
IL-10*
Human E. coli 1064-ILB
HSC Human
Sf21 (stably transfected)
217-ILB
Human HEK293 11178-IL
Human E. coli 1064-IL
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https://www.bio-techne.com/t/flt-3-ligand-flt3l/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/g-csf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/gdf-5-bmp-14/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/gdnf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/gm-csf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/hb-egf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/hgf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/ifn-gamma/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/igf-i-igf-1/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/il-2/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/il-3/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/il-4/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/il-6/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/il-7/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/il-10/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/reagents/proteins/stem-cell-proteins?pdfSource=true_proteins-for-stem-cell-culture
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Key: ESC Embryonic Stem Cells iPSC Induced Pluripotent Stem Cells HSC Hematopoietic Stem Cells MSC Mesenchymal Stem Cells NSC Neural Stem Cells
* GMP-grade proteins are available for these molecules.
Molecule Species Source Catalog # Stem Cell Type
IL-11 Human CHO 10836-IL ESC, iPSC, HSC
IL-15* Human E. coli BT-015 HSC
IL-21* Human E. coli 8879-IL HSC
M-CSF* Human E. coli 216-MC
HSC Human CHO 216-MCC
beta-NGF Human NS0 256-GF NSC
Nodal Human E. coli 3218-ND ESC, iPSC
Noggin* Human NS0 6057-NG ESC, iPSC, NSC
NT-4 Human Sf21 (baculovirus) 268-N4 NSC
PDGF-BB* Human E. coli 220-BB ESC, iPSC, MSC, NSC
R-Spondin 1 Human CHO 4645-RS ESC, iPSC
R-Spondin 3 Human CHO 3500-RS ESC, iPSC
SCF* Human E. coli BT-SCF
ESC, iPSC, HSC Human HEK293 11010-SC
Sonic Hedgehog/Shh* Human HEK293 8908-SH
ESC, iPSC, MSC, NSC Human E. coli 1845-SH
TGF-b1* Human HEK293 7754-BH ESC, iPSC, MSC
TGF-b2 Human NS0 302-B2 MSC
TGF-b3 Human CHO 8420-B3 MSC
Thrombopoietin* Human E. coli 288-TPE
ESC, iPSC, HSC Human NS0 288-TPN
VEGF* Human E. coli BT-VEGF
ESC, iPSC, HSC, NSC Human Sf21 (baculovirus) 293-VE
Wnt-3a* Human CHO 5036-WN ESC, iPSC, HSC
Wnt-5a Human/Mouse CHO 645-WN ESC, iPSC
Wnt-5b Human CHO 7347-WN MSC
Wnt-10b Human CHO 7196-WN MSC
https://www.bio-techne.com/t/il-11/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/il-15/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/il-21/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/m-csf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/beta-ngf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/nodal/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/noggin/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/nt-4/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/pdgf-bb/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/r-spondin-1/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/r-spondin-3/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/scf-c-kit-ligand/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/sonic-hedgehog-shh/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/tgf-beta-1/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/tgf-beta-2/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/tgf-beta-3/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/thrombopoietin-tpo/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/vegf/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/wnt-3a/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/wnt-5a/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/wnt-5b/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/t/wnt-10b/proteins-enzymes?pdfSource=true_proteins-for-stem-cell-culture
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No Protein
A B
Activin A
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FIGURE 1. Differentiation of Human Embryonic Stem Cells in Media Supplemented with R&D Systems Recombinant Human Activin A. BG01V human embryonic stem cells were (A) cultured in media without any protein added or (B) differentiated into endoderm using media supplemented with Recombinant Human Activin A (R&D Systems, Catalog # 338-AC). Differentiation into endoderm was confirmed by positive staining for Claudin-6 (red) and Sox17 (green) using the Mouse Anti-Human Claudin-6 Monoclonal Antibody (R&D Systems, Catalog # MAB3656) and the Goat Anti-Human Sox17 Polyclonal Antibody (R&D Systems, Catalog # AF1924), respectively. BG01V human embryonic stem cells are licensed from ViaCyte, Inc.
FIGURE 2. R&D Systems Recombinant Human Noggin Displays Higher Activity than Leading Competitors’ Noggin Proteins and Minimal Lot-to-Lot Variability. (A) The bioactivity of R&D Systems Recombinant Human Noggin (Catalog # 6057-NG; purple line) or recombinant human Noggin from a top competitor (teal line) was determined by assessing the ability of the proteins to inhibit alkaline phosphatase production induced by 50 ng/mL Recombinant Human BMP-4 (R&D Systems, Catalog # 314-BP) in the ATDC5 mouse chondrogenic cell line. In the presence of 50 ng/mL Recombinant Human BMP-4, the ED50 for this effect for R&D Systems Recombinant Human Noggin was approximately 30-fold greater than the top competitor’s Noggin protein. (B, C) BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm
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using a 3-day incubation in 25 ug/mL of R&D Systems Recombinant Human Noggin (Lot 1, Lot 2; Catalog # 6057- NG) or recombinant human Noggin from two different competitors (Competitor 1, Competitor 2). Control cells were incubated in media without Noggin (No Noggin). The cells were stained for the early ectoderm marker, Otx2, and the neuroectoderm marker, SOX1. (B) Representative images of Otx2 (red), SOX1 (green), and DAPI (blue) staining in embryonic stem cells differentiated with Noggin from R&D Systems or Noggin from Competitor 2. (C) SOX1+ clusters were quantified under each of the indicated culture conditions. Cells treated with R&D Systems Recombinant Human Noggin showed an increase in SOX1+ cells compared to both the untreated and competitor- treated cells. R&D Systems Recombinant Human Noggin showed consistent differentiation across the lots tested. BG01V human embryonic stem cells were licensed from ViaCyte, Inc.
https://www.bio-techne.com/services/bulk-quotes?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-mouse-rat-activin-a-protein_338-ac?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-claudin-6-antibody-342927_mab3656?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-claudin-6-antibody-342927_mab3656?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-sox17-antibody_af1924?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-noggin-protein_6057-ng?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-noggin-protein_6057-ng?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-bmp-4-protein_314-bp?pdfSource=true_proteins-for-stem-cell-culture file: https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-noggin-protein_6057-ng?pdfSource=true_proteins-for-stem-cell-culture
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A. KRT5 (Basal Cells) / p63 (Basal Cells) PDPN (Alveolar Type I cells) Muc5a (Goblet Cells)/Sox2 Muc5a (Goblet Cells)/Sox2B. C. D.
FIGURE 4. Culture and Characterization of Adult Stem Cell-derived Human Lung Organoids. Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes Recombinant Human R-Spondin 1 (R&D Systems, Catalog # 4645-RS), Recombinant Human Noggin (R&D Systems, Catalog # 6057-NG), Recombinant Human FGF-7 (R&D Systems, Catalog # 251-KG), and Recombinant Human FGF-10 (R&D Systems, Catalog # 345-FG), along with the other reagents listed in the lung organoid expansion medium recipe in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (R&D Systems, Catalog # AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (R&D Systems, Catalog # AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (R&D Systems, Catalog # MAB2018; red). All samples were counterstained with DAPI (Tocris Bioscience, Catalog # 5748; blue).
FIGURE 3. Culture and Characterization of iPSC- derived Human Intestinal Organoids. iPSC-derived human intestinal organoids were embedded in Cultrex™ UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and cultured in intestinal organoid culture medium, which includes Recombinant Human Wnt-3a (R&D Systems, Catalog # 5036-WN), Recombinant Human Noggin (R&D Systems, Catalog # 6057-NG), Recombinant Human R-Spondin 1 (R&D Systems, Catalog
A. B. # 4645-RS), Recombinant Human EGF (R&D Systems, Catalog # 236-EG), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/ Mouse/Rat Vimentin Monoclonal Antibody (R&D Systems, Catalog # MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Tocris Bioscience, Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF748; green) and a Mouse Anti- Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Tocris Bioscience, Catalog # 5748; blue).
Culture of iPSC-derived Intestinal Organoids or Adult Stem Cell- derived Lung Organoids using R&D Systems Proteins
https://www.bio-techne.com/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-r-spondin-1-protein_4645-rs?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-r-spondin-1-protein_4645-rs?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-noggin-protein_6057-ng?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-kgf-fgf-7-protein_251-kg?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-fgf-10-protein_345-fg?pdfSource=true_proteins-for-stem-cell-culture https://www.rndsystems.com/resources/protocols/human-lung-organoid-culture-protocol?pdfSource=true_proteins-for-stem-cell-culture https://www.rndsystems.com/resources/protocols/human-lung-organoid-culture-protocol?pdfSource=true_proteins-for-stem-cell-culture https://www.novusbio.com/products/cytokeratin-5-antibody-ep1601y_nb110-56916?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-p63-tp73l-antibody_af1916?pdfSource=true_proteins-for-stem-cell-culture https://www.novusbio.com/products/podoplanin-antibody-811_nb600-1015?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-p63-tp73l-antibody_af1916?pdfSource=true_proteins-for-stem-cell-culture https://www.novusbio.com/products/muc5ac-antibody-45m1_nbp2-15196?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-mouse-rat-sox2-antibody-245610_mab2018?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/fluorescent-probes-dyes/dapi_5748?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-wnt-3a-protein_5036-wn?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-wnt-3a-protein_5036-wn?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-noggin-protein_6057-ng?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-noggin-protein_6057-ng?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-r-spondin-1-protein_4645-rs?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-egf-protein-cf_236-eg?pdfSource=true_proteins-for-stem-cell-culture https://www.rndsystems.com/resources/protocols/human-intestinal-organoid-culture-protocol?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-mouse-rat-vimentin-antibody-280618_mab2105?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-mouse-rat-vimentin-antibody-280618_mab2105?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-mouse-desmin-antibody_af3844?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-mouse-desmin-antibody_af3844?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/fluorescent-probes-dyes/dapi_5748?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-mouse-e-cadherin-antibody_af748?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-mouse-e-cadherin-antibody_af748?pdfSource=true_proteins-for-stem-cell-culture https://www.novusbio.com/products/muc2-antibody-mlp-842_nbp2-44431?pdfSource=true_proteins-for-stem-cell-culture https://www.novusbio.com/products/muc2-antibody-mlp-842_nbp2-44431?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/fluorescent-probes-dyes/dapi_5748?pdfSource=true_proteins-for-stem-cell-culture
8
GMP-Grade Proteins for Regenerative Medicine and Cell Therapies GMP-grade proteins are manufactured under guidelines that allow for their use as ancillary materials in cell therapy manufacturing processes. They undergo extensive quality control testing and come with comprehensive documentation and full transparency and traceability of source and manufacturing system. This allows cell therapy manufacturers to be confident that they are using a consistent, safe, and traceable supply of reagents.
Documentation
Regulatory Guidelines Followed
Quality Control Testing
Satisfied Clients
GMP products manufactured, tested, and released under an ISO 9001:2015 and ISO 13485:2016 certified quality management system, lot-to-lot consistency, materials traceability, employee training and documentation, equipment maintenance and monitoring records, Drug Master Files, and more.
GMP proteins are manufactured in compliance of the applicable sections of the World Health Organization:
1. USP Chapter <1043>, Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.
2. Ph. Eur. General Chapter 5.2.12, Raw Materials of Biological Origin for the Production of Cell-base and Gene Therapy Medicinal Products
Mass spectrometry, HPLC, SDS-PAGE, endotoxin, presence of host cell content, adventitious agents, and more.
Clients include more than 300 pharmaceutical and biotech companies and we regularly welcome audits of our facilities.
Learn More About Our Animal-Free Proteins | bio-techne.com/reagents/proteins/animal-free-proteins
Animal-Free Preclinical Proteins In addition to our research-grade proteins, Bio-Techne also offers Animal-free preclinical proteins and GMP-grade proteins. Our Animal-free preclinical proteins are made in the same facility using the same processes as our GMP- grade proteins. Since the proteins are the same, they make excellent reagents for research and process development and allow for a smooth transition from preclinical work to clinical manufacturing. Animal-free proteins are purified and manufactured in dedicated, controlled access, animal-free laboratories using equipment and media that are certified as animal-free. We have a strict definition of animal-free and at no point in the production process are these proteins exposed to any animal components or byproducts. The catalog numbers for our Animal-free preclinical proteins are listed in the product table alongside the corresponding GMP-grade proteins.
https://www.bio-techne.com/reagents/proteins/animal-free-proteins?pdfSource=true_proteins-for-stem-cell-culture
9
Animal-Free Preclinical and GMP-Grade Proteins Table 2.
* DMF have been filed for these GMP Proteins. GMP-grade IL-2, IL-7, and IL-15 are available through our joint venture partnership with ScaleReady | scaleready.com
Protein (Human; Source: E. coli)
Animal-Free GMP-Grade Protein (Catalog #)
Animal-Free Preclinical Protein (Catalog #)
Betacellulin BT-BTC-GMP* BT-BTC-AFL
BMP-4 314E-GMP AFL314E
EGF 236-GMP* AFL236
FGF basic (145 aa) 3718-GMP AFL3718
Flt-3 Ligand/FLT3L 308E-GMP* AFL308E
GM-CSF 215-GMP AFL215
IFN-g 285-GMP* AFL285
IGF-I 291-GMP AFL291
LR3 IGF-I 8335D-GMP
IL-1b/IL-1F2 201-GMP AFL201
IL-2 BT-002-GMP* BT-002-AFL
IL-3 203-GMP AFL203
IL-4 BT-004-GMP Coming Soon
BT-004-AFL
IL-6 206-GMP AFL206
IL-7 BT-007-GMP* BT-007-AFL
IL-10 1064-GMP AFL1064
IL-15 BT-015-GMP* BT-015-AFL
IL-21 8879-GMP* AFL8879
M-CSF 216-GMP AFL216
PDGF-AA 221-GMP AFL221
PDGF-BB 220-GMP AFL220
SCF/c-kit Ligand BT-SCF-GMP BT-SCF-AFL
Shh N-terminus 1314-GMP
Shh (C2411) N-terminus
1845-GMP AFL1845
Thrombopoietin 288E-GMP AFL288 Coming Soon
TNF-a 210-GMP AFL210
VEGF BT-VEGF-GMP BT-VEGF-AFL
https://www.scaleready.com/?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-betacellulin-gmp-protein-cf_bt-btc-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-betacellulin-protein-animal-free_bt-btc-afl?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-bmp-4-gmp-protein-cf_314e-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-bmp-4-animal-free-protein_afl314e?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-egf-gmp-protein-cf_236-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-egf-animal-free-protein_afl236?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-fgf-basic-fgf2-145-aa-gmp-protein-cf_3718-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-fgf-basic-fgf2-bfgf-animal-free-protein_afl3718?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-flt-3-ligand-flt3l-gmp-protein-cf_308e-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-flt-3-ligand-flt3l-animal-free-protein_afl308e?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-gm-csf-gmp-protein-cf_215-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-gm-csf-animal-free-protein_afl215?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-ifn-gamma-gmp-protein-cf_285-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-ifn-gamma-animal-free-protein_afl285?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-igf-i-igf-1-gmp-protein-cf_291-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-igf-i-igf-1-animal-free-protein_afl291?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-lr3-igf-i-igf-1-gmp-protein-cf_8335d-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-1-beta-il-1f2-gmp-protein-cf_201-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-1-beta-il-1f2-animal-free-protein_afl201?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-2-gmp-protein-cf_bt-002-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-2-protein-animal-free_bt-002-afl?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-3-gmp-protein-cf_203-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-3-animal-free-protein_afl203?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-4-protein-animal-free_bt-004-afl?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-6-gmp-protein-cf_206-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-6-animal-free-protein_afl206?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-7-gmp-protein-cf_bt-007-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-7-protein-animal-free_bt-007-afl?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-10-gmp-protein-cf_1064-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-10-animal-free-protein_afl1064?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-15-gmp-protein-cf_bt-015-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-15-protein-animal-free_bt-015-afl?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-21-gmp-protein-cf_8879-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-21-animal-free-protein_afl8879?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-m-csf-gmp-protein-cf_216-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-m-csf-animal-free-protein_afl216?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pdgf-aa-gmp-protein-cf_221-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pdgf-aa-animal-free-protein_afl221?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pdgf-bb-gmp-protein-cf_220-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pdgf-bb-animal-free-protein_afl220?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-scf-gmp-protein-cf_bt-scf-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-scf-protein-animal-free_bt-scf-afl?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-sonic-hedgehog-shh-n-terminus-gmp-cf_1314-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-sonic-hedgehog-shh-c24ii-n-term-gmp-cf_1845-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-sonic-hedgehog-shh-c24ii-animal-free-cf_afl1845?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-thrombopoietin-gmp-protein-cf_288e-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-tnf-alpha-gmp-protein-cf_210-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-tnf-alpha-animal-free-protein_afl210?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-vegf-165-gmp-protein-cf_bt-vegf-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-vegf-165-protein-animal-free_bt-vegf-afl?pdfSource=true_proteins-for-stem-cell-culture
10
Learn More About Our GMP Proteins | bio-techne.com/gmp-products/gmp-proteins
* DMF have been filed for these GMP Proteins.
R&D Systems GMP-grade proteins are intended for use as ancillary materials in GMP manufacturing of investigational or marketed clinical products, such as cell therapy, gene therapy, tissue-engineered products, combination products, or other Advanced Therapy Medicinal Products. They are not therapeutic products or excipient and are not suitable for direct administration to humans.
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-
A. B.
iNK Fold Expansion iNK purity
20
40
60
0
100
120
80
20
40
60
0
80
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FIGURE 5. Assessment of the Expansion, Purity, and Killing Capacity of Human iPSC-derived Natural Killer Cells Differentiated with R&D Systems GMP-grade Cytokines. Based on a protocol by Zhu, H. and D. Kaufman (2019) Methods Mol. Biol., human induced pluripotent stem cells (iPSCs) were grown in the presence of R&D Systems GMP-grade Recombinant Human VEGF165 (Catalog # BT-VEGF-GMP), GMP-grade Recombinant Human SCF (Catalog # 255B-GMP), GMP-grade Recombinant Human BMP-4 (Catalog # 314E-GMP), and GMP Y-27632 dihydrochloride (Catalog # TB1254- GMP) and then differentiated in the presence of GMP-grade Recombinant Human IL-3 (Catalog # 203-GMP), GMP-grade Recombinant Human IL-7 (Catalog # BT-007-GMP), GMP-grade Recombinant Human IL-15 (Catalog # BT-015-GMP), GMP- grade Recombinant Human SCF (Catalog # 255B-GMP), and GMP-grade Recombinant Human Flt-3 Ligand (Catalog # 308E-GMP). (A) Thirty days after initiating cell differentiation from iPSCs, the average fold expansion and purity of iNK cells were respectively determined by hemacytometer and flow cytometry using an Alexa Fluor® 405-conjugated Mouse Anti- Human CD3 epsilon Monoclonal Antibody (Catalog # FAB100V) and an Alexa Fluor® 647-conjugated Rabbit Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # FAB24086R). The experiment was performed in triplicate. (B) To assess iNK cell functionality, the cells were expanded for three days and a killing assay was performed by adding the iNK cells to fluorescently-labeled K-562 target cells at a 2:1 effector:target ratio. Loss of fluorescence, indicative of target cell killing, was quantitatively measured after three more days using an Incucyte® Live Cell Analysis System. The killing assay was performed in triplicate. The x-axis shows biological replicates (N=3) and the error bars show technical replicates (N=3). Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Incucyte® is a registered trademark of Essen Instruments, Inc., Ann Arbor, MI.
Table 3.
Protein (Human) Source Catalog #
Activin A CHO 338-GMP*
BMP-2 CHO 355-GMP
GDF-8/Myostatin NS0 788-GMP
GDNF NS0 212-GMP
HGF NS0 294-GMP
KGF/FGF-7 E.coli 251-GMP
Noggin NS0 3344-GMP
TGF-b1 CHO 240-GMP
Wnt-3a CHO 5036-GMP
Additional GMP-grade Proteins Available from Bio-Techne
There are some instances when a protein requires production in a eukaryotic system to maintain activity. This may be due to protein folding or post-translational modifications that can only be accomplished by making the protein in a eukaryotic cell line. These GMP-grade proteins, which are not considered to be animal-free, are listed in the table below. Whenever a GMP-grade protein cannot be produced in an animal-free process, it is always clearly indicated on our website.
https://www.bio-techne.com/gmp-products/gmp-proteins?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-vegf-165-gmp-protein-cf_bt-vegf-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-vegf-165-gmp-protein-cf_bt-vegf-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-scf-gmp-protein-cf_255b-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-bmp-4-gmp-protein-cf_314e-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/small-molecules-peptides/y-27632-dihydrochloride_tb1254-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-3-gmp-protein-cf_203-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-7-gmp-protein-cf_bt-007-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-7-gmp-protein-cf_bt-007-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-15-gmp-protein-cf_bt-015-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-scf-gmp-protein-cf_255b-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-scf-gmp-protein-cf_255b-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-flt-3-ligand-flt3l-gmp-protein-cf_308e-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-cd3epsilon-alexa-fluor-405-conjugated-antibody-ucht1_fab100v?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-cd3epsilon-alexa-fluor-405-conjugated-antibody-ucht1_fab100v?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-ncam-1-cd56-alexa-fluor-647-conjugated-antibody-2524c_fab24086r?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/antibodies/human-ncam-1-cd56-alexa-fluor-647-conjugated-antibody-2524c_fab24086r?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-mouse-rat-activin-a-gmp-protein-cf_338-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-bmp-2-gmp-protein-cf_355-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-mouse-rat-gdf-8-myostatin-gmp-protein-cf_788-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-gdnf-gmp-protein-cf_212-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-hgf-ns0-expressed-gmp-protein-cf_294-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-kgf-fgf-7-gmp-protein-cf_251-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-noggin-fc-chimera-gmp-protein-cf_3344-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-tgf-beta-1-gmp-protein-cf_240-gmp?pdfSource=true_proteins-for-stem-cell-culture https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-wnt-3a-gmp-protein-cf_5036-gmp?pdfSource=true_proteins-for-stem-cell-culture
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Explore Proteins for Stem Cell Culture | bio-techne.com/reagents/proteins/stem-cell-proteins
https://www.bio-techne.com/services/custom-protein-services?pdfSource=true_immune-cell-isolation-culture https://www.bio-techne.com/reagents/proteins/stem-cell-proteins?pdfSource=true_proteins-for-stem-cell-culture
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