R&D Systems Luminex Portfolio Brochure (2).PDF
Immunoassays
R&D Systems Luminex Portfolio
®
™
Instruments, Assays, and Software
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Luminex Instruments and R&D Systems Assays
From screening biomarkers for further evaluation to validating results for drug development, multiplex immunoassays offer several critical performance advantages. The ability to measure multiple targets in a single assay allows researchers to detect hundreds of analytes in a single run while being judicious with precious sample volumes.
As one of the most trusted platforms for high-level protein multiplexing, R&D Systems Luminex assays are designed to maximize multiplexing capacity and flexibility while maintaining assay specificity. Utilizing Luminex xMAP® microparticle technology, Luminex assays from R&D Systems are rigorously validated for accuracy, reproducibility, and lot-to-lot consistency.
R&D Systems™ has a complete solution to optimize productivity across your Luminex®
workflow.
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Validation and Testing • Antibody pairs are selected and tested to confirm the
parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analyte within biological samples
• R&D Systems Luminex Assays are validated for use with cell culture supernatants, plasma, or serum.
• Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.
Luminex Assays by R&D Systems R&D Systems Luminex Assays are ideal for researchers needing to evaluate multiple analytes for trends in protein expression in disease states or following treatment. Validated and tested for optimum performance, R&D Systems Luminex Assays are designed to optimize multiplexing capabilities while delivering consistent and accurate results.
• Choose from a rapidly expanding menu of over 450 analytes
• Select kits designed for human, mouse, rat, non-human primate, and porcine analytes
• Run a multiple analyte assay in 3–3.5 hours
• Assay multiple analytes in a small sample volume (< 50 μL)
• Choose from three flexible formats
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Luminex 200 Instrument System
Luminex FLEXMAP 3D Instrument System
Luminex xMAP INTELLIFLEX
System
Luminex xMAP INTELLIFLEX DR-SE
System
Number of targets Multiplex capacity
Up to 100 Up to 500 Up to 500 Up to 500
Read time for 96 well plate
~40 minutes ~20 minutes ~20 minutes ~20 minutes
Read time for 384 well plate
N/A ~75 minutes ~75 minutes ~75 minutes
Dynamic range 3.5 logarithmic units 4.5 logarithmic units ≥5.5 logarithmic units ≥5.5 logarithmic units
Microtiter wells 96 96 and 384 96 and 384 96 and 384
Dimensions (W x D x H)
25.3” x 23.5” x 12.5” 64cm x 60cm x 32.5cm
23” x 25.7” x 18” 58.4cm x 63.5cm x
45.7cm
23” x 24” x 30” 58.4cm x 61cm x 76.2cm
23” x 24” x 30” 58.4cm x 61cm x 76.2cm
Touchscreen N/A N/A Yes Yes
Automated startup N/A N/A Yes Yes
Reporter laser 532 nm (green) 532 nm (green) 532 nm (green) 532 nm (green) and
405 nm (violet)
Dual reporter readout
N/A N/A N/A Yes
Barcode reader for calibration and verification reagents
N/A N/A Yes Yes
Find the Luminex multiplexing system perfect for your laboratory’s needs.
Luminex Instrumentation Luminex instruments offer proven precision and reliability, making them among the most widely used multiplexing platforms in the market. Using a mixture of color-coded beads pre-coated with capture antibodies, powerful xMAP technology offers the ability to bind up to 50 different analyte targets in solution. Upon completion of an R&D Systems Luminex assay, beads are analyzed with a dedicated dual- laser flow-based detection instrument. From the Luminex 200 to the advanced xMAP INTELLIFLEX, choose the Luminex instrument that best meets your unique application requirements.
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Quantist Software Quantist Luminex data analysis software helps you extract valuable insights from data complexity. Use this software to evaluate the quality of your standards, controls, and unknowns. Evaluate the long-term consistency of your Luminex data by using Quantist to make inter-assay comparisons. Import CSV files from any Luminex instrument and export ready-to-graph, Excel files.
Processor 3.0 GHz i7 6th gen
Ram 8 GB
Hard Drive 256 GB
Ports 1-USB 1-NIC
OS Windows 10 Pro 64bit
Screen Resolution 1600x900 24 bit color
Quantist Minimum Software Requirements
• Easily exclude individual wells or aggregates
• Remove low bead counts with a single click
• Assign or update well assignments in the Luminex 96-well plate
• Export optimized data for graphing
Try free for 30 days | bio-techne.com/reagents/luminex/luminex-software-quantist
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NEW Luminex xMAP INTELLIFLEX The xMAP INTELLIFLEX system is an advanced multiplexing platform that delivers accurate and reliable protein measurement results. Featuring a second reporter laser (405 nm) alongside the traditional reporter channel, the INTELLIFLEX system enables the detection of two parameters on a single bead, allowing you to maximize your data points and the number of samples you can process. Designed for a 96- or 384-well high throughput format, the INTELLIFLEX system can save substantial time compared to a single-plex or lower-throughput assay.
INTELLIFLEX Features
xMAP INTELLIFLEX DR-SE System
All features of the xMAP INTELLIFLEX System plus:
• Dual reporter system Second violet (405 nm) laser allows you to monitor two parameters per analyte.
• Side-eject door Enables end-to-end automation to maximize throughput.
• 5.5 logarithmic units of dynamic range Flexibility for multiplex targets minimizes the need to dilute samples to fall within the range of the assay.
• Integrated touchscreen PC Integrated touchscreen enables space savings and convenient and intuitive setup.
• Built-in barcode reader Faster setup and convenient tracking system, particularly for large experiments.
• Automated startup, shutdown & maintenance routines Increase your productivity with walk-away routines.
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xMAP® INTELLIFLEX RUO xMAP® INTELLIFLEX DR-SE RUO®
Part Number INTELLIFLEX-RUO INTELLIFLEX-DRSE-RUO
Performance
Multiplexing Capability Up to 500 individual analytes per well Up to 500 individual analytes per well; Up to 2 parameters per analyte
Typical Dynamic Range ≥5.5 logs ≥5.5 logs ≥4.5 logs (second reporter cahnnel*)
Sensitivity Commercially availalbe kits deliver sensitivity as low as sub-picogram level
General
Weight 54.4 kg (120lbs)
System Warm-Up 30 min
Operating Temperature 15-30°C (59-85°F)
Operating System Microsoft Windows 10 IoT Enterprise LTSC
Barcode Reader For importing target values from the xMAP INTEILLIFLEX Calibration and Performance Verification Kits
Reagents Included xMAP® INTELLIFLEX Performance Verification Kit xMAP® INTELLIFLEX Calibration Kit xMAP® Sheath Fluid PLUS, 20 L
Fluidics
Sheath Pressure 8-13 psi
Sample Injection Rate 2 μL/sec
Sample Uptake Volume 10-200 μL
Optics
Lasers 1 Classification laser 1 Reporter laser
1 Classification laser 2 Reporter lasers*
Classification Laser Excitiation
638 nm
Reporter Laser 1 (RP1) Excitation
532 nm
Reporter Laser 2 (RP2) Excitation*
n/a 405 nm
RP1 Detector 565-589 nm
RP2 Detector n/a 421-441 nm
* The second reporter channel is only available in the xMAP INTELLIFLEX DR-SE RUO model.
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Luminex 200 The Luminex 200 is the most popular installed instrument for basic multiplexing protein needs.
• Simultaneously multiplex up to 100 analytes in a single microplate well
• Delivers fast and cost-effective bioassay results on many assay formats
• Calibrated using a calibration and a verification kit, each providing 25 uses
Luminex 200 System with xPONENT 4.2
Part Number LX200-XPON-RUO
Core Instrument Luminex 200 flow-based bead reader with: Sheath Delivery System; X-Y Platform for 96-well plates; Sample probe-needle; 1L sheath bottle, 1.2L Waste bottle; Sample probe height adjustment kit; Automated Maintenance Plate; Heater Block (96 well plate); Documentation
Available Power Cords (pick one)
USA, Australia, Brazil, Denmark, Germany, Sweden, France, Belgium, Spain, Switzerland, Israel, UK, Italy, Japan, India,China, S. Africa, Argentina, Korea, Taiwan
Barcode Scanner Optional, at no charge with purchase of new system
Software Included xPONENT 3.1 (3 seats) Allele-call module (3 seats) Remote Web Monitoring module (3 seats)
Optional Software (extra charges apply)
LIS integration module Automation interface module Basic Security Module 21 CFR Part 11 Module (supersedes Basic Security) Additional seats
Reagents Included LX200-CAL-K25, qty 1 (separately shipped) LX200-CON-K25, qty 1 (separately shipped) xMAP Sheath Fluid (20L), qty 1 (ships with LX200)
Desktop PC with Win 7 32-bit, US English & xPONENT 3.1
Dell Optiplex with 2 Port PCI RS232 Serial Adapter Card Dell Warranty Transfer Dell 17” LCD monitor with soundbar Dell Keyboard & mouse
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FLEXMAP 3D® The FLEXMAP 3D is an advanced and highly versatile multiplexing platform. Fast read time, compatibility with 96- and 384-well plates, and ease of integration with front-end automation systems make the FLEXMAP 3D the platform of choice for high-throughput applications.
• Multiplexing: Up to 500 analytes in a single sample volume
• Dynamic Range (Typical): >4.5 logs
• Read Time: 96-well plate in ≤ 20 min (up to 144,000 tests/ hour); 384-well plate in ≤ 75 min (up to 153,600 tests/hour)
• Daily Start-Up/Shut-Down: ≤ 30 min
FLEXMAP 3D xPONENT 4.2 System
Part Number FLEXMAP-3D-RUO
Core Instrument FLEXMAP 3D flow based bead reader with: Sample probe-needle; Off plate reagent block; Waste container; Sample probe height adjustment tool; Barcode Scanner; Heater Block 96-well; Heater Block 386- well; Documentation
Available Power Cords (pick one)
USA, Australia, Brazil, Denmark, Germany, Sweden, France, Belgium, Spain, Switzerland, Israel, UK, Italy, Japan, India, China, S. Africa, Argentina, Korea, Taiwan, Pakistan
Software Included xPONENT 4.2 (3 seats), Allele-call module (3 seats) Remote Web Monitoring module (3 seats)
Optional Software (extra charges apply)
LIS integration module Automation interface module Basic Security Module 21 CFR Part 11 Module (supersedes Basic Security) Additional seats
Reagents Included F3D-CAL-K25, qty 1 (separately shipped) F3D-PVER-K25, qty 1 (separately shipped) Sheath Fluid (20L) qty 1 (Ships with FLEXMAP 3D)
Desktop PC with Win 7 32-bit, US English
PC, Desktop, USFF, WIN 7 with XPON 4.2 Dell OptiPlex 7010 “Ultra Small Form Factor” Dell Warranty Transfer Arm Mounted ELO 19” Touch Screen Monitor AX2010 USB Stereo Speaker System Keyboard & Mouse
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xMAP® Instrument Reagents
Magnetic Plate Separator The Luminex Magnetic Plate Separator, LU-MAG-01, is a modified plate magnet intended to separate a 96-well plate of MagPlex Microspheres during assay preparation steps. The side clasps on this magnet have been specially designed to hold a microplate securely to the magnet, enabling the user
Instrument Service Plans
Reagent Pack Size Part Number
MAGPIX MAGPIX Drive Fluid, 4 × 750 mL containers MAGPIX Calibration Kit MAGPIX Performance Verification Kit
4 pack 25 uses 25 uses
MPXDF-4PK MPX-CAL-K25 MPX-PVER-K25
Luminex 100/200 (all versions)
Sheath Fluid Sheath Concentrate Pack (20x)
20 L 1L
40-50000 40-75680
Luminex 100/200 (xPONENT only)
Luminex 100/200 Calibration Kit Luminex 100/200 Performance Verification Kit
25 uses 25 uses
LX200-CAL-K25 LX200-CON-K25
FLEXMAP 3D
Sheath Fluid Sheath Concentrate Pack (20x) FLEXMAP 3D Calibration Kit FLEXMAP 3D Performance Verification Kit
20L 1L 25 uses 25 uses
40-50000 40-75680 F3D-CAL-K25 F3D-PVER-K25
INTELLIFLEX
xMAP® INTELLIFLEX Performance Verification Kit RUO xMAP® INTELLIFLEX Calibration Kit RUO xMAP® Sheath Fluid PLUS RUO xMAP® Sheath Concentrate PLUS
20 uses 20 uses 20L 1L
IFX-PVER-K20 IFX-CAL-K20 40-50021 40-50023
Luminex 200, FLEXMAP 3D, and INTELLIFLEX
Number of visits for emergency repair Unlimited
Emergency repair costs included in plan All Costs Included
Preventative maintenance visits per year 1–2
On-site response time (business days) 1–2
Priority scheduling for service requests Included
Product updates and hardware modifications Included
Software support Included
Unlimited 24 ×7 × 365 remote support Included (Unlimited, during business hours - Japan)
On-site assay support, as needed (Luminex Assays Only) Not Included
Learn more | bio-techne.com/instruments/luminex
to quickly invert and decant it over a waste container. Similar to the method used in an ELISA, this manual plate washing method has been shown to effectively remove nearly all of the supernatant from every well on the plate, with virtually no bead carry-over.
https://www.bio-techne.com/p/luminex-instrument/96-well-magnetic-plate-separator_lu-mag-01?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/instruments/luminex?pdfSource=true_luminex-assays-instruments
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Ordering Your R&D Systems Luminex Assays The R&D Systems Luminex Assay Online Ordering Tool is designed to help you choose the right Luminex Assay for your unique research needs.
• Save your selected Luminex Assay configuration for easy re-ordering
• View how the selection of each assay specification changes the final kit options
• Find pricing options for premixed or user mixed High Performance Assays
• Alter your assay selections at any time with the tool’s easy navigation
• Get access to ordering or technical support via phone and email
Ordering assays online is easy | bio-techne.com/ luminex-assay-customization-tool
Custom Luminex Services Employ our assay experts and our extensive in-house collection of antibodies and proteins to build your specific custom multiplex panel. Custom services may include performance optimization of analytes from our menu of over 450 analytes, or the creation of an assay for a biomarker that we don’t currently offer in any product categories, or even the evaluation of specific sample types that our kits were not initially tested for. Expedite your project by putting our experience and reagents to work for you.
• Create multiplex assays for existing or new analytes
• Develop assays for your unique specifications
• Validate for your specific sample types
• Supply agreements available
Learn more online bio-techne.com/services/custom-luminex-services
Bio-Techne Academy The Luminex Learning Plan at the Bio-Techne Academy has all the training you need toget up and running with your Luminex instrument, assays, and Quantist Analysis software. The course includes modules on Luminex technology, installation, ordering and running R&D Systems assays, how to analyze data, instrument maintenance protocols and troubleshooting. Align the team with courses ranging from a quick refresher to new employee training.
Self-register to get more! academy.bio-techne.com/learn
ACADEMY THE ONLINE EXPERIENCE
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Luminex Assay Offerings
https://www.bio-techne.com/luminex-assay-customization-tool?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/luminex-assay-customization-tool?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/services/custom-luminex-services?pdfSource=true_luminex-assays-instruments https://academy.bio-techne.com/learn?pdfSource=true_luminex-assays-instruments https://academy.bio-techne.com/learn?pdfSource=true_luminex-assays-instruments
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R&D Systems Luminex High Performance Assays
Correlated with the gold standard Quantikine™ ELISA kits, our Luminex High Performance Assay panels are optimized with custom diluents and extensively validated to ensure consistent and accurate results. Pre-defined multiplex panels are designed using key analytes from chosen research areas.
• Our most accurate and precise bead-based multianalyte profiling kits.
• High sensitivity panels available for low abundance proteins.
• Large XL Cytokine panels for detecting 38-46 analytes.
• Flexible panel options from configurable premixed kits, user mix, and off-the-shelf fixed panels
Learn more | bio-techne.com/reagents/luminex- assays/luminex-high-performance-assays
Figure 2. The Human XL Cytokine Performance Panel, FCSTM18B, detects the inflammatory response of Cytokine-Release Syndrome (CRS) biomarkers. Unstimulated and stimulated PBMCs were tested for multiple analytes to measure the different levels that are seen in CRS. The Human XL Cytokine Performance Panel’s high sensitivity and superior accuracy offers the most reliable method of measuring CRS-associated inflammatory cytokines.
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20,000
0
60,000
100,000
140,000
Analyte
IFNg TNFa IL-10 IL-1ra MIP-1a MIP-1B IL-1ra
p g
/m L
Unstimulated (before) Stimulated (after)
Unstimulated vs. Stimulated PBMCs
Figure 1. Luminex Assays undergo testing to ensure that they detect natural proteins in parallel with the recombinant protein standard. A dilution curve detecting natural or recombinant standard CXCL16 is shown. Parallel detection of natural and recombinant standard proteins ensure that the concentration within a biological sample can be accurately determined in comparison to the recombinant protein used to generate the standard curve.
R&D Systems Luminex Discovery Assays
Personalize your multiplex immunoassay panel using Luminex Discovery Assays. Create your panel of up to 50 analytes with the largest customizable menu on the market. Explore our large catalog of human, mouse, rat, and porcine targets. All assays can be performed in 3.5 hours or less.
• Most flexible panel building on the market
• Custom built panels of up to 50 analytes
• Panels come pre-mixed for easy set-up
• Validated and tested for optimum performance
Learn more | bio-techne.com/reagents/luminex- assays/luminex-discovery-assays
M F
I
CXCL16 Standard Natural CXCL16
CXCL16 (pg/mL)
1 101
102
103
104
101
102 103
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Choose Your R&D Systems Luminex Assay Format
Luminex Discovery Assay Luminex High Performance Assay
Species Available
Human
Mouse
Rat
Primate
Porcine
Sample Volume Required
µL Sample ≤ 25 - 50 ≤ 25 - 50
Multiplex Analyte Capacity
Magnetic 50 User-Selected User-Selected from Panel, or Fixed Panel
Validated Sample Types
Serum
Plasma
Cell Culture Supernates See Datasheet**
Milk See Datasheet**
Saliva See Datasheet**
Urine See Datasheet**
Run Time Hours 3–3.5 2.5–5
Kit Preparation Premixed
End-user Mixed
Assay Validation
Mean Sensitivity < the low standard* ≤ 3/4 the low standard
Intra-assay Precision < 20% < 15%
Intra-assay Precision < 30% < 17%
Recovery (Individual Samples)
N/A 70–130%
Assay Linearity Dose-Dependent Decrease in Values 1:2–1:8
Linearity (Individual Samples)
Dose-Dependent Decrease in Values 70–130%
Analytes Available
Human > 350 >135
Mouse > 108 45
Rat 17 0
Primate 0 36
Porcine 20 0
*Mean sensitivity details | bio-techne.com/luminex-assay-customization-tool **For more information | bio-techne.com/reagents/luminex-assays/luminex-high-performance-assays
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R&D Systems Luminex® High Performance Assays Fixed Panels
IFN-alpha IFN-gamma IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2
IL-1ra/IL-1F3 IL-2 IL-3 IL-4
IL-6 IL-7 IL-9 IL-10
IL-15 IL-33 VEGF
Human Cytokine Panel 15-Plex, LKTM011B
CCL2/JE/MCP-1 CCL3/MIP-1 alpha
CCL4/MIP-1 beta CCL5/RANTES
CCL11/Eotaxin CXCL1/GRO alpha
CXCL10/IP-10/CRG-2 IL-8/CXCL8
Human Chemokine Panel 8-Plex, LKTM012B
CCL3/MIP-1 alpha CCL19/MIP-3 beta CD40 Ligand/TNFSF5 CXCL2/GRO beta EGF FGF basic/FGF2/bFGF
Flt-3 Ligand/FLT3L G-CSF Granzyme B IFN-beta IL-3 IL-8/CXCL8
IL-9 IL-17E/IL-25 IL-33 PD-L1/B7-H1 PDGF-AA PDGF-AB/BB
TGF-alpha TRAIL/TNFSF10 VEGF
Human Growth Factor Panel 21-Plex, LKTM013B
CCL2/JE/MCP-1 CCL3/MIP-1 alpha CCL4/MIP-1 beta CD40 Ligand/TNFSF5 CXCL10/IP-10/CRG-2 GM-CSF Granzyme B
IFN-alpha IFN-gamma IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-1ra/IL-1F3 IL-2 IL-4
IL-6 IL-8/CXCL8 IL-9 IL-10 IL-12 p70 IL-13 IL-15
IL-17/IL-17A IL-33 PD-L1/B7-H1 TNF-alpha
Human Immunotherapy Panel 25- Plex, LKTM010B
GM-CSF IFN-gamma IL-1 beta/IL-1F2 IL-2
IL-4 IL-5 IL-6 IL-9
IL-10 IL-12 p70 IL-13 TNF-alpha
TNF-beta
Human Th1/Th2 Panel 13-Plex, LKTM008B
CCL20/MIP-3 alpha CD40 Ligand/TNFSF5 GM-CSF IFN-gamma IL-1 beta/IL-1F2
IL-2 IL-4 IL-5 IL-6 IL-9
IL-10 IL-12 p70 IL-13 IL-15 IL-17/IL-17A
IL-17E/IL-25 IL-33 TNF-alpha
Human Th9/Th17/Th22 Panel 18-Plex, LKTM009B
TIMP-1 TIMP-2 TIMP-3 TIMP-4
Human TIMP Panel 4-Plex, LKTM003
CCL2/JE/MCP-1 CCL3/MIP-1 alpha CCL4/MIP-1 beta CCL5/RANTES CCL11/Eotaxin
CCL19/MIP-3 beta CCL20/MIP-3 alpha CD40 Ligand/TNFSF5 CXCL1/GRO alpha CXCL2/GRO beta
CXCL10/IP-10/CRG-2 EGF FGF basic/FGF2/bFGF Flt-3 Ligand/FLT3L G-CSF
GM-CSF Granzyme B IFN-alpha 2/IFNA2 IFN-beta IFN-gamma
Human XL Cytokine Panel 46-Plex, LKTM014B
https://www.bio-techne.com/p/luminex-assays/human-cytokine-luminex-performance-assay-15-plex-fixed-panel_lktm011b?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/p/luminex-assays/human-chemokine-luminex-performance-assay-8-plex-fixed-panel_lktm012b?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/p/luminex-assays/human-growth-factor-luminex-performance-assay-21-plex-fixed-panel_lktm013b?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/p/luminex-assays/human-immunotherapy-luminex-performance-assay-25-plex-fixed-panel_lktm010b https://www.bio-techne.com/p/luminex-assays/human-th1-th2-luminex-performance-assay-13-plex-fixed-panel_lktm008b?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/p/luminex-assays/human-th9-th17-th22-luminex-performance-assay-18-plex-fixed-panel_lktm009b?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/p/luminex-assays/human-timp-luminex-performance-assay-4-plex-fixed-panel_lktm003?pdfSource=true_luminex-assays-instruments https://www.bio-techne.com/p/luminex-assays/human-xl-cytokine-luminex-performance-assay-46-plex-fixed-panel_lktm014b?pdfSource=true_luminex-assays-instruments
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R&D Systems Luminex® High Performance Assays Fixed Panels
IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-1ra/IL-1F3 IL-2 IL-3 IL-4 IL-5
IL-6 IL-7 IL-8/CXCL8 IL-9 IL-10 IL-12 p70 IL-13
IL-15 IL-17/IL-17A IL-17E/IL-25 IL-33 PD-L1/B7-H1 PDGF-AA PDGF-AB/BB
TGF-alpha TNF-alpha TNF-beta TRAIL/TNFSF10 VEGF
Human XL Cytokine Panel 46-Plex, LKTM014B (continued)
BAFF/BLyS/TNSFS13B CCL2/JE/MCP-1 CCL3/MIP-1 alpha CCL4/MIP-1 beta CCL5/RANTES CCL11/Eotaxin CCL19/MIP-3beta Chitinase 3-like 1 CXCL1/KC CXCL10/IP-10/CRG-2 EGF FGF-basic
Flt-3 ligand G-CSF GDF-15 GM-CSF I-CAM IFN-gamma IL- 1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-1ra/IL-1F3 IL-2 IL-3 IL-4
IL-5 IL-6 IL-7 IL-9 IL-10 IL-11 IL-12 p70 IL-13 IL-16 IL-17A IL-18 IL-21
IL-27 IL-31 LDL R LIF LIX M-CSF TIMP-1 TNF-alpha VEGF
Mouse XL Cytokine Panel 45-Plex, LKTM015
BAFF/BLyS CCL20/MIP-3 alpha CD14
CD27/TNFRSF7 CXCL13/BLC/BCA-1 gp130
IL-2 Ra CD25/IL-2R alpha TNF RII/TNFRSF1B
Human Biomarker Panel A, Premix FCSTM13, User Mix LBAM000
CCL2/MCP-1 CCL3/MIP-1 alpha CCL4/MIP-1 beta CCL5/RANTES CXCL5/ENA-78 CXCL8/IL-8
FGF basic G-CSF GM-CSF IFN-gamma IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2
IL-1ra/IL-1F3 IL-2 IL-4 IL-5 IL-6 IL-10
IL-17 TNF-alpha Thrombopoietin/Tpo VEGF
Human Cytokine Panel A, Premix FCSTM03, User Mix LUHM000
CXCL8/IL-8 GM-CSF IFN-gamma
IL-1 beta/IL-1F2 IL-2 IL-4
IL-5 IL-6 IL-10
IL-12 p70 TNF-alpha VEGF
Human High Sensitivity Cytokine Panel A, Premix FCSTM09, User Mix LHSCM000
GM-CSF IFN-g IL-1b IL-2 IL-5
IL-6 IL-7 IL-13 IL-15 IL-17A
IL-17F IL-22 IL-23 IL-31 IL-33
IL-36 beta TNF-alpha
Human High Sensitivity Cytokine Panel B, Premix FCSTM14, User Mix LBHS000
R&D Systems Luminex® High Performance Assays
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16
R&D Systems Luminex® High Performance Assays
TGF- beta1 TGF- beta2 TGF- beta3
Multi-species TGF-b Panel, Premix FCSTM17, User Mix LTGM00
BAFF/BLyS/TNSFS13B CCL2/JE/MCP-1 CCL3/MIP-1 alpha CCL4/MIP-1 beta CCL5/RANTES CCL11/Eotaxin
CCL19/MIP-3beta Chitinase 3-like 1 CXCL1/KC CXCL10/IP-10/CRG-2 EGF FGF-basic
Flt-3 ligand G-CSF GDF-15 GM-CSF I-CAM IFN-gamma
IL- 1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-1ra/IL-1F3 IL-2 IL-3 IL-4
Mouse XL Cytokine Performance Panel, Premix FCSTM20, User Mix LMXL000
MMP-1 MMP-2 MMP-3
MMP-7 MMP-8 MMP-9
MMP-10 MMP-12 MMP-13
EMMPRIN
Human MMP Panel, Premix FCSTM07, User Mix LMPM000
C-Peptide CCL2/JE/MCP-1 Ghrelin
GLP-1 Glucagon IFN-gamma
IL-6 Insulin Leptin/OB
Pancreatic Polypeptide/PP Peptide YY TNF-alpha
Human Metabolic Panel, Premix FCSTM19
Adiponectin/Acrp30 C-Reactive Protein/CRP CCL2/MCP-1
Comp. Factor D/Adipsin IL-6 IL-10
Leptin/OB Resistin Serpin E1/PAI-1
TNF-a
Human Obesity Panel, Premix FCSTM08, User Mix LOBM000
CCL2/MCP -1 CCL3/MIP-1 alpha CCL4/MIP-1 beta CCL5/RANTES CCL11/Eotaxin CCL19/MIP-3 beta CCL20/MIP-3 alpha CD40 Ligand CX3CL1/Fractalkine CXCL1/GRO alpha CXCL2/GRO beta CXCL10/IP-10
EGF FGF basic/FGF-2 Flt-3 Ligand G-CSF GM-CSF Granzyme B IFN- alpha IFN- beta IFN- gamma IL-1 alpha IL-1 beta IL-1ra
IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-8/CXCL8 IL-9 IL-10 IL-12 p70 IL-13 IL-15
IL-17A IL-17E/IL-25 IL-33 PD-L1/B7-H1 PDGF-AA PDGF-BB TGF- alpha TNF- alpha TNF-beta TRAIL VEGF
Human XL Cytokine Panel, Premix FCSTM18B, User Mix LUXLM000B
Clusterin CXCL10/IP-10/CRG-2
Cystatin C Lipocalin-2/NGAL
Osteopontin (OPN) TFF3
Human Kidney Biomarker Panel, Premix FCSTM16, User Mix LHK000
CCL17/TARC
CD27/TNFRSF7
CD28
CD40/TNFRSF5
CD25/IL-2R alpha
CTLA-4
CXCL5/ENA-78
HVEM/TNFRSF14
IFN-gamma
LAG-3
OX40 Ligand/TNFSF4
PD-L1/B7-H1
PD-L2/B7-DC
TIM-3
Human Immuno-Oncology Panel Performance Panel, Premix FCSTM24
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17
R&D Systems Luminex® Discovery Assays
Human Discovery Assay, Catalog # LXSAHM
4-1BB/TNFRSF9/CD137 ADAMTS13 Adiponectin/Acrp30 Aggrecan AgRP/ART ALCAM/CD166 Aldehyde Dehydrogenase 1-A1/ALDH1A1 alpha 1-Microglobulin alpha 2-Macroglobulin alpha-Fetoprotein/AFP alpha-N-acetylglucosamini- dase/NAGLU alpha-Synuclein Angiogenin Angiopoietin-1 Angiopoietin-2 Angiopoietin-like Protein 3/ ANGPTL3 Angiopoietin-like Protein 4/ ANGPTL4 Angiopoietin-like Protein 6/ ANGPTL6 APP APRIL/TNFSF13 Atrial Natriuretic Peptide/ ANP B7-H3 BAFF/BLyS/TNFSF13B BCMA/TNFRSF17 BDNF beta 2-Microglobulin beta-NGF BMP-10 BMP-2 BMP-4
BMP-7 BMP-9 CA125/MUC16 CA15-3/MUC-1 Calbindin D Carbonic Anhydrase IX/CA9 Cathepsin S CCL1/I-309/TCA-3 CCL11/Eotaxin CCL13/MCP-4 CCL14/HCC-1/HCC-3 CCL15/MIP-1 delta CCL17/TARC CCL18/PARC CCL19/MIP-3 beta CCL2/JE/MCP-1 CCL20/MIP-3 alpha CCL21/6Ckine CCL22/MDC CCL23/MPIF-1 CCL24/Eotaxin-2/MPIF-2 CCL25/TECK CCL26/Eotaxin-3 CCL27/CTACK CCL28 CCL3/MIP-1 alpha CCL4/MIP-1 beta CCL5/RANTES CCL7/MCP-3/MARC CCL8/MCP-2 CD117/c-kit CD14 CD163 CD23/Fc epsilon RII CD25/IL-2R alpha CD27/TNFRSF7
CD30/TNFRSF8 CD31/PECAM-1 CD40 Ligand/TNFSF5 CD40/TNFRSF5 CD44 CEACAM-1/CD66a CEACAM-5/CD66e Chemerin Chitinase 3-like 1 Coagulation Factor III/Tissue Factor Coagulation Factor XIV/ Protein C Collagen IV alpha 1 Complement Component C2 Complement Component C5a Complement Component C9 Complement Factor D/ Adipsin Contactin-1 C-Peptide C-Reactive Protein/CRP Cripto CX3CL1/Fractalkine CXCL1/GRO alpha/KC/ CINC-1 CXCL10/IP-10/CRG-2 CXCL11/I-TAC CXCL13/BLC/BCA-1 CXCL14/BRAK CXCL16 CXCL2/GRO beta/MIP-2/ CINC-3
CXCL4/PF4 CXCL5/ENA-78 CXCL6/GCP-2 CXCL7/NAP-2 CXCL9/MIG Cystatin C DcR3/TNFRSF6B D-dimer Dkk-1 DPPIV/CD26 DR3/TNFRSF25 EGF EMMPRIN/CD147 Endocan/ESM-1 Endoglin/CD105 Endostatin Endothelin-1 Enolase 2/Neuron-specific Enolase ENPP-2/Autotaxin EN-RAGE/S100A12 EpCAM/TROP1 EphA2 ErbB2/Her2 ErbB3/Her3 E-Selectin/CD62E FABP4/A-FABP Fas Ligand/TNFSF6 Fas/TNFRSF6/CD95 Ferritin Fetuin A/AHSG FGF acidic/FGF1 FGF basic/FGF2/bFGF FGF-13 FGF-23 Fibroblast Activation Protein
R&D Systems Luminex® High Performance Assays
BDNF CCL2/JE/MCP-1 CCL5/RANTES CCL11/Eotaxin CCL20/MIP-3 alpha CD40 Ligand/TNFSF5 CXCL2/GRO beta/MIP-2/ CINC-3 CXCL10/IP-10/CRG-2
CXCL11/I-TAC CXCL13/BLC/BCA-1 FGF basic/FGF2/bFGF G-CSF GM-CSF Granzyme B IFN- alpha IFN- beta IFN gamma
IL-1 beta/IL-1F2 IL-4 IL-5 IL-6 IL-7 IL-8/CXCL8 IL-10 IL-12 p70 IL-13
IL-15 IL-17/IL-17A IL-21 PD-L1/B7-H1 PDGF-AA PDGF-BB TGF- alpha TNF- alpha VEGF
Non Human Primate XL Cytokine Panel, Premix FCSTM21
IL-5 IL-6 IL-7 IL-9 IL-10 IL-11
IL-12 p70 IL-13 IL-16 IL-17A IL-18 IL-21
IL-27 IL-31 LDL R LIF LIX M-CSF
TIMP-1 TNF-alpha VEGF
Mouse XL Cytokine Performance Panel, Premix FCSTM20, User Mix LMXL000 (continued)
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18
R&D Systems Luminex® Discovery Assays
alpha/FAP Fibronectin Flt-3 Ligand/FLT3L Follistatin-like 1/FSTL1 Follistatin-related Gene Protein/FLRG Furin Galectin-1 Galectin-3 Galectin-3BP/MAC-2BP Galectin-9 Gas6 G-CSF GDF-15 GDNF GITR/TNFRSF18 GM-CSF gp130 Granzyme A Granzyme B Growth Hormone HB-EGF HE4/WFDC2 HGF HGFR/c-MET HMW Adiponectin/Acrp30 HTRA2/Omi ICAM-1/CD54 IFN-alpha IFN-beta IFN-gamma IFN-gamma R1/CD119 IGFBP-1 IGFBP-2 IGFBP-3 IGFBP-4 IGFBP-6 IGFBP-rp1/IGFBP-7 IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-1 RI IL-1 RII IL-10 IL-11 IL-12 p70 IL-12/IL-23 p40 IL-13 IL-15 IL-16 IL-17/IL-17A IL-17C IL-17E/IL-25 IL-18/IL-1F4 IL-19 IL-1ra/IL-1F3 IL-2 IL-21
IL-23 IL-27 IL-28A/IFN-lambda 2 IL-28B/IFN-lambda 3 IL-3 IL-31 IL-33 IL-34 IL-36 beta/IL-1F8 IL-4 IL-4R alpha IL-5 IL-6 IL-6R alpha IL-7 IL-8/CXCL8 Insulin ITIH4 Kallikrein 3/PSA Kallikrein 5 Kallikrein 6/Neurosin Lactoferrin LBP Leptin R Leptin/OB LIF LIGHT/TNFSF14 Lipocalin-2/NGAL LRG1 L-Selectin/CD62L Lumican Lymphotoxin-alpha/ TNF-beta MAdCAM-1 MBL MCAM/CD146 M-CSF M-CSF R/CD115 Mesothelin MFG-E8 MIA MICA MICB Midkine MIF MMP-1 MMP-12 MMP-13 MMP-2 MMP-3 MMP-7 MMP-8 MMP-9 MSP/MST1 Myeloperoxidase/MPO Myoglobin N-Cadherin
NCAM-1/CD56 Nectin-4 Nephrin Neuregulin-1 beta 1/NRG1 beta 1 Neuropilin-1 NT-3 NT-4 Oncostatin M/OSM Osteoactivin/GPNMB Osteopontin/OPN Osteoprotegerin/TNFRS- F11B Park7/DJ-1 PBEF/Visfatin PDGF-AA PDGF-AB PDGF-BB PDGF-CC PDGF-DD PD-L1/B7-H1 Pentraxin 2/SAP Pentraxin 3/TSG-14 Periostin/OSF-2 PLA2G7/PAF-AH/Lp-PLA2 PlGF Procalcitonin Pro-Collagen I alpha 1 Progranulin/PGRN Prolactin Properdin Proprotein Convertase 9/ PCSK9 Protein S/PROS1 Proteinase 3/Myeloblastin/ PRTN3 P-Selectin/CD62P RAGE/AGER RBP4/Retinol-Binding Protein 4 Reg3A Relaxin-2 Renin Resistin ROBO4 S100A8 S100A9 S100B SCF/c-kit Ligand SCGF/CLEC11a Serpin A10/ZPI Serpin A12 Serpin A4/Kallistatin Serpin A7/TBG Serpin B3/SCCA1 Serpin C1/Antithrombin-III Serpin E1/PAI-1
Serpin F1/PEDF SHBG SLPI SOST/Sclerostin SPARC SP-D ST2/IL-33R Stanniocalcin 1/STC-1 Syndecan-1/CD138 Syndecan-4 TACI/TNFRSF13B Tau Tenascin C TFF3 TFPI TfR (Transferrin R) TGF-alpha Thrombomodulin/BDCA-3 Thrombopoietin/Tpo Thrombospondin-2 Thymidine Kinase 1 Tie-2 TIM-1/KIM-1/HAVCR TIMP-1 TNF RI/TNFRSF1A TNF RII/TNFRSF1B TNF-alpha Total Inhibin TRACP/PAP/ACP5 TRAIL R2/TNFRSF10B TRAIL R3/TNFRSF10C TRAIL/TNFSF10 TRANCE/TNFSF11/RANK L TREM-1 Troponin I TSLP UCH-L1/PGP9.5 ULBP-1 ULBP-2/5/6 ULBP-3 ULBP-4/RAET1E uPAR u-Plasminogen Activator (uPA)/Urokinase Uromodulin Uteroglobin/SCGB1A1 VAP-1/AOC3 VCAM-1/CD106 VEGF VEGF-C VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4 Vitamin D BP vWF-A2
Human Discovery Assay, Catalog # LXSAHM (continued)
https://www.bio-techne.com/p/luminex-assays/human-luminex-discovery-assay_lxsahm?pdfSource=true_luminex-assays-instruments
19
CXCL2/GRO beta/MIP-2/ CINC-3 CXCL3/GRO gamma/ CINC-2/DCIP-1 GM-CSF
ICAM-1/CD54 IFN-gamma IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-10
IL-13 IL-18/IL-1F4 IL-2 IL-4 IL-6
L-Selectin/CD62L TIMP-1 TNF-alpha VEGF
Rat Discovery Assay, Catalog # LXSARM
Adiponectin/Acrp30 Angiopoietin-2 BAFF/BLyS/TNFSF13B beta-NGF C1qR1/CD93 CCL11/Eotaxin CCL12/MCP-5 CCL19/MIP-3 beta CCL2/JE/MCP-1 CCL20/MIP-3 alpha CCL21/6Ckine CCL22/MDC CCL3/MIP-1 alpha CCL4/MIP-1 beta CCL5/RANTES CCL7/MCP-3/MARC CCL8/MCP-2 Chitinase 3-like 1 Complement Factor D/ Adipsin C-Reactive Protein/CRP CXCL1/GRO alpha/KC/ CINC-1 CXCL10/IP-10/CRG-2 CXCL12/SDF-1 alpha CXCL13/BLC/BCA-1 CXCL16 CXCL2/GRO beta/MIP-2/
CINC-3 Cystatin C Dkk-1 DPPIV/CD26 EGF EGFR EMMPRIN/CD147 Endoglin/CD105 FABP4/A-FABP Fas Ligand/TNFSF6 FGF basic/FGF2/bFGF FGF-21 G-CSF GDF-15 GM-CSF Granzyme B Haptoglobin HGF ICAM-1/CD54 IFN-gamma IGFBP-1 IGFBP-3 IGF-I/IGF-1 IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-10 IL-12 p70 IL-13
IL-16 IL-17/IL-17A IL-17E/IL-25 IL-2 IL-27 IL-3 IL-33 IL-4 IL-5 IL-6 IL-6R alpha IL-7 LDLR LIX M-CSF MMP-12 MMP-2 MMP-3 MMP-8 MMP-9 Nephrin Oncostatin M/OSM Osteopontin/OPN Osteoprotegerin/TNFRS- F11B PDGF-AA PDGF-AB PDGF-BB
Periostin/OSF-2 PlGF-2 Podocalyxin Prolactin Proprotein Convertase 9/ PCSK9 P-Selectin/CD62P Renin Resistin S100A8 S100A9 Serpin E1/PAI-1 SP-D Syndecan-1/CD138 Thrombospondin-4 TIM-1/KIM-1/HAVCR TIMP-1 TIMP-4 TNF RI/TNFRSF1A TNF RII/TNFRSF1B TNF-alpha TRANCE/TNFSF11/RANK L TWEAK/TNFSF12 uPAR VEGF VEGFR2/KDR/Flk-1
Mouse Discovery Assay, Catalog # LXSAMSM
CD31/PECAM-1 CD34 C-Reactive Protein/CRP GM-CSF IFN-gamma
IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-10 IL-12 IL-18/IL-1F4
IL-1ra/IL-1F3 IL-2 IL-4 IL-6 IL-8/CXCL8
MMP-1 PDGF-BB Serpin E1/PAI-1 TNF-alpha
Porcine Discovery Assay, Catalog # LXSAPM
R&D Systems Luminex® Discovery Assays
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