Recombinant Proteins

2

Setting the Standard in Proteins for Almost 40 years

Trusted by Research In a survey of more than 800 publications

covering proteins in areas of cancer, immunology,

neuroscience and stem cell research, 36% cited use

of Bio-Techne proteins, greater than any other single

protein manufacturer.

Leading the Way in Bioscience Bio-Techne recombinant proteins were compared

with those from other manufacturers in side-by-side

bioassays. Over half of the 43 tested Bio-Techne

proteins exhibited 1.5-fold or higher bioactivity,

compared to competitor proteins.

All of our R&D Systems-brand proteins by Bio-Techne are produced by scientists – for scientists, so we understand that producing recombinant proteins is about generating the reliable tools that researchers need to thrive. We manufacture more than 95% of our proteins. It give us complete control over all aspects of production, so nothing becomes a Bio-Techne protein that does not meet our industry-leading specifications.

36% Bio-Techne

1% 1%

1% 2%

2% 2%

2% 3%

3%

4%

4%

5%

34%

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First Class Products and Services

The Four Pillars of Bio-Techne Quality

We understand that experimental outcomes can be limited

by the performance of your protein. That is why the focus

on quality starts at the birth of each Bio-Techne protein.

During product development our optimization process

involves analysis of protein sequence, expression system,

and formulation to ensure we provide proteins with the

best possible bioactivity and performance.

We are proud that Bio-Techne experience and

dedication to protein development and manufacturing

is unmatched in the life science industry.

Our quality commitment does not stop with our

products Wherever you are in the world, you can

access our highly trained technical service staff and the

scientists responsible for protein development, so we

can answer any questions you might have.

1 BIOAC TIVIT Y

3 STABILIT Y

2 CONSISTENCY

4 PURIT Y

Biological activity assays, based on the available scientific literature,

are developed for each protein we produce. Each new lot has to pass our

strict QC activity parameters before it is released to the market.

We constantly monitor the condition of our proteins to check for long

term performance. Our proteins are shipped lyophilized or in specially optimized formulations, so each

recombinant protein reaches you in perfect condition.

You can be sure of the reliability of our proteins because of our rigorous QC and production standards. Each new

lot is compared to the previous for bioactivity, purity and endotoxin level.

Be confident that the results obtained in your experiments are not due to contaminants. The majority of our

proteins have >95% purity and have guaranteed industry-leading endotoxin

level of ≤0.1 EU/μg (established by LAL assay).

Learn More // bio-techne.com/about/quality

https://www.bio-techne.com/about/quality?pdfSource=true_recombinany-protein-brochure

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Covering the Research Areas You Need At Every Step

STEM CELL S IMMUNOLOGY NEUROSCIENCE

Culture and Characterization of Adult Stem Cell- derived Human Lung Organoids. Adult stem cells isolated from human lung biopsy tissue

were embedded in Cultrex UltiMatrix RGF Basement

Membrane Extract (Catalog # BME001-05) and cultured

for 20-60 days in lung organoid expansion medium,

which includes R-Spondin 1 (Catalog # 4645-RS),

Noggin (Catalog # 6057-NG) FGF-7 (Catalog # 251-

KG), and FGF-10 (Catalog # 345-FG), along with the

other reagents listed in the lung organoid expansion

medium recipe in the human lung organoid culture

protocol. Lung organoids were able to differentiate and

exhibit markers for various cell types of the lung. Lung

organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Catalog

NB110-56916; green) and a Goat Anti-Human p63/

TP73L Polyclonal Antibody (Catalog # AF1916; red)

to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Catalog #

NB600-1015; green) to visualize alveolar type I cells and

a Goat Anti-Human p63/TP73L Polyclonal Antibody

(Catalog # AF1916; red) to visualize basal cells, and

(C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Catalog # NBP2-15196; green) to visualize Goblet cells

and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal

Antibody (Catalog # MAB2018; red). All samples were

counterstained with DAPI (Catalog # 5748; blue).

Affinity Measurements and Binding Kinetics of the PD-1:PD-L1 Interaction by Surface Plasmon Resonance. Sensorgram data of captured Avi-tag Biotinylated

PD-L1 His tag (Catalog # AVI9049) binding to PD-1 His

tag (Catalog # 8986-PD). The corresponding overlaid

kinetic fits with the residual plot shown below. The

concentration of PD-1 His-tag ranged from 3.2 nM to

13.2 μM. The corresponding steady state affinity fit is

shown below. The experiment was performed on a

BiacoreT200, GE Healthcare.

Recombinant Mouse Meteorin CATALOG # 3475-MN Immunofluorescent image of E16-E18 stage rat cortical

neurons treated with Meteorin (10 ug/uL) to induce neurite

outgrowth. Cells were stained with a nuclear stain (Blue,

DAPI) and beta-III Tubulin (Red, Cat# MAB1195).

R e

sp o

n se

50

60

70

RU

PD-1: PD-L1 Sesorgrams

Time

KD=1.122uM 40

30

20

10

0

-10 0 20 40 60 80 100 120

S

R e

sp o

n se

60

70

RU

PD-1:PD-L1 Steady State Affinity Fit

Concentration

KD=1.528 uM 50

40

20

30

10

0 2e-60 4e-6 6e-6 8e-6 1e-5 1.2e-5 1.4e-5

Time

40

30

20

5

-10 0-20

-20

20 40 60 80 100 120

S

What is important to you in a recombinant protein? Is it the species, the activity, available size, expression system or documentation?

Every research question is unique and so requires a unique solution. That is why

the range of Bio-Techne recombinant proteins give you the choices to tailor the right

protein solution to your research.

By listening to the scientists we serve, Bio-Techne produces the protein tools

that are the most useful to you.

From immunology to apoptosis, neuroscience to cancer research — our protein

catalogue spans the widest range of research areas. This ensures that Bio-Techne

is your partner for all recombinant protein needs — whatever your research goals.

https://www.bio-techne.com/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-r-spondin-1-protein_4645-rs?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-noggin-protein_6057-ng?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-kgf-fgf-7-protein_251-kg?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-kgf-fgf-7-protein_251-kg?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-fgf-10-protein_345-fg?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/antibodies/human-p63-tp73l-antibody_af1916?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/antibodies/human-mouse-rat-sox2-antibody-245610_mab2018?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/fluorescent-probes-dyes/dapi_5748?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-his-tag-avi-tag-protein-cf_avi9049?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-his-tag-avi-tag-protein-cf_avi9049?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pd-1-his-tagged-protein-cf_8986-pd?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pd-1-his-tagged-protein-cf_8986-pd?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-mouse-meteorin-protein_3475-mn?pdfSource=true_recombinany-protein-brochure

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The Format You Need — For Your Research and Clinical Applications

As research projects evolve – from basic research to pre-clinical applications, the requirements for your reagents also evolve. The availability of key reagents in the format you need can influence the pace of your discovery.

With over 30 years of experience in producing the most trusted natural and

recombinant proteins, Bio-Techne produces proteins in a range of formats that give

you choice and flexibility whatever your requirements.

Research Grade Designed for the researcher, we offer a wide selection

of cytokines, growth factors, enzymes, and drug

targets. Often these are available with different tags,

biotinylation, or fluorescent conjugation.

Animal-Free Manufacturing Conditions For those interested in pursuing clinical applications in cell

therapy or minimizing ethical and safety issues associated

with animal-derived reagents. These proteins are made in

the same facility and have the same specifications as our

GMP proteins allowing for smooth transitions to clinical

manufacturing.

Good Manufacturing Practices (GMP) - Grade Meeting regulatory guidelines for use in clinical

manufacturing in immune cell and regenerative medicine-

based cell therapies. To find out more about our wide range

of GMP-grade proteins see page 6.

Learn More // bio-techne.com/reagents/proteins

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https://www.bio-techne.com/reagents/proteins?pdfSource=true_recombinany-protein-brochure

6

Full Document and Traceability All proteins are produced in our ISO-9001:2008 and ISO 13485:2003 facilities. All

are supported by extensive documentation with quality and regulatory oversight.

Drug master files are available.

Ideal for Transitional Research We also offer Research Use Only (RUO) Animal-Free proteins produced in the same animal-

free GMP facility. These RUO animal-free proteins share the same biological activities as our

GMP-grade proteins, easing the process of transition from pre-clinical to clinical applications.

GMP-Grade Proteins

For Clinical Applications These reagents meet regulatory guidelines as ancillary materials for cell

therapy manufacturing. They are designed with consistency and scalability

in mind. GMP proteins come with full quality and regulatory support and

Bio-Techne understands the importance of partnership as you advance

through clinical trials and commercialization.

Totally Animal-Free Bio-Techne uses a strict definition of animal-free. Some may label a protein

animal-free even when there are animal components in the manufacturing

process. Be confident when we call something animal-free.

• Animal-Free RUO proteins are made exclusively in E. coli

• Produced in dedicated, controlled-access, animal-free laboratories

• Animal-free media and equipment used in the manufacturing process

• Proteins are NOT exposed to potential contamination by animal

components at any point during the production process

• Animal-free statements required for incoming materials

Benefits of Our GMP Facilities • Entirely dedicated to cytokines and growth factors used in cell

therapy manufacturing

• All animal-free facility

• Capacity to meet supply needs now and in the future

• ISO 5/7/8 cleanrooms for the entire production process

• ISO 9001:2015 and ISO 13485:2016 Quality Management Systems

• Additional Facilities, Quality & Regulatory Information

Learn More //

bio-techne.com/gmp-

products/gmp-proteins

6

https://www.bio-techne.com/gmp-manufacturing-facility?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/gmp-products/gmp-capabilities?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/gmp-products/gmp-proteins?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/gmp-products/gmp-proteins?pdfSource=true_recombinany-protein-brochure

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GMP-Grade Proteins: Consistency Data Highlighted with G-Rex

CD4+ and CD8+ T cells isolated from a human donor were cultured in a G-Rex® 6M Well Plate (ScaleReady) containing media

with one of three lots of GMP-grade IL-7 and IL-15 (Catalog # BT-007-GMP and Catalog # BT-015-GMP). The average (A) cell

yield and (B) cell viability for each lot of cytokines were determined by flow cytometry on day 14. This data shows around a

60-fold expansion of the cells after G-Rex culture with GMP-grade IL-7 and IL-15.

T Cell Yield and Viability After G-Rex® Culture in Media Containing GMP IL-7 & IL-15

A. B.

G-Rex® is a registered trademark of Wilson Wolf Corporation. This data is not yet available on the Bio-Techne website.

Lot 2Lot 1 Lot 3

To ta

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ll N

u m

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V ia

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% )

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Quality is Key Bio-Techne understands that consistency is a key characteristic of cytokines and growth factors used in clinical

manufacturing. New GMP lots must not only meet the indicated specifications for activity, but also must match the activity of

master lots maintained by Bio-Techne. This lot-to-lot consistency is highlighted below in a T cell isolation assay using a G-Rex

cell culture device from Wilson Wolf, part of our ScaleReady joint venture with Fresenius Kabi.

Learn More // https://www.scaleready.com/product/gmp-cytokines-for-cell-therapy-manufacturing/

https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-7-gmp-protein-cf_bt-007-gmp?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-il-15-gmp-protein-cf_bt-015-gmp?pdfSource=true_recombinany-protein-brochure https://www.scaleready.com/product/gmp-cytokines-for-cell-therapy-manufacturing?pdfSource=true_recombinany-protein-brochure

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Bulk Quantities

Custom Protein Services

Quality Protein on the Scale You Need Ordering Bio-Techne recombinant

proteins in bulk delivers the same

quality proteins you know and expect,

but at the perfect price and scale for

your research. We have the ability to

scale up protein production to meet

your large scale needs. Ask us about

bulk availability in either liquid or

lyophilized formats. With our new GMP

facility, large lot sizes minimize the

need for bridging lots.

Apply Our Experience to Your Research When your research demands unique proteins or specialist

solutions, Bio-Techne can be your partner to achieve your aims.

Since 1985, Bio-Techne has produced proteins to meet the highest

development and purification standards and as part of Bio-Techne,

we share expertise spanning laboratory research, pre-clinical

studies, and reagents for manufacturing diagnostic tests.

Harnessing our decades of product development experience,

our specialist protein solutions and custom services provide the

unique tools and reagents.

From Cloning to Purification From the initial cloning to the choice of expression systems and

purification method - we can cater to any level of specification

Harness our specialist animal free and GMP grade protein

facilities for your custom protein production.

Protein Modification and Processing We can tailor in vitro modification and processing to meet your needs. Avi-tagged biotinylation, PEGylation, proteolytic cleavage

and fluorokines are just some of the modifications available.

Expertise and Communication All projects start with an extensive consultation to capture all the

specific project and performance requirements.

A team is assembled from our expert research and development

scientists, manufacturing engineers, and project management staff.

Together, our expert team will work with you to deliver the protein

that meets you required specifications.

Expert Bioactivity Testing No company matches the bioassay experience of Bio-Techne.

We have over 900 established bioassays available to test proteins,

small molecules and antibodies.

Learn More // bio-techne.com/services/custom-protein-services

Learn More //

bio-techne.com/services/bulk-quotes

https://www.bio-techne.com/services/custom-protein-services?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/services/bulk-quotes?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/services/bulk-quotes?pdfSource=true_recombinany-protein-brochure

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Constantly Striving for Great Performance We know that scientific progress doesn’t stand still, and in

developing research solutions neither do we.

From the daily challenges of the laboratory, changing

reagent demands and emerging scientific trends, we are

constantly challenged by our customers to solve their

research demands.

Providing the best reagents and solutions to meet your

challenges is at the heart of Bio-Techne mindset. Using our

expertise and experience we develop innovative protein

reagent and kits to help you solve your important scientific

questions.

Protein Process Engineering From innovative cell culture and purification techniques

to engineering proteins with enhanced characteristics,

we employ industry leading protein biochemistry to

create innovative new products.

Bioactivity —So What? You may think that all recombinant proteins are

created equally, but using poorly performing proteins

in your experiments could be wasting your precious

research budget.

Better performing protein means you need to use

less, therefore saving you money.

Learn More // bio-techne.com/reagents/proteins

http://www.bio-techne.com/reagents/proteins?pdfSource=true_recombinany-protein-brochure

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Human T lymphocytes were treated

with the indicated concentrations of

either unlabeled PD-L1/B7-1 Fc Chimera

(Catalog # 156-B7; green line) or Avi-tag

Biotinylated PD-L1/B7-H1 Fc Chimera

(Catalog # AVI156; orange line). IL-2

secretion was measured in cell culture

supernatants using the Human IL-2

Quantikine® ELISA Kit (Catalog # D2050).

The similarity in the activities of the

two proteins highlights that the Avi-tag

biotinylated protein is fully functional.

Specialized Proteins Avi-Tag Biotinylation and Fluorescent Conjugation

Bringing You the Best Solutions

All Bio-Techne proteins and products are born with the demands of our customers in mind, as they are developed by experienced researchers.

To make sure we bring only the best protein products to life, we use the latest

science and technology, which ensures they are relevant and useful scientific tools.

Biotinylated Proteins and Fluorescent-labeled Proteins (Fluorokines)

10-2 10-1 100

0.3

0.2

0.1

0.4

0.5

Recombinant Human PD-L1/B7-H1 Fc Chimera (µg/mL)

IL -1

S e

cr e

ti o

n (

M e

a n

O .D

.)

0.6

Human PD-L1 Fc, Avi-tag

Human PD-L1 Fc, No Tag

Unlabeled and Avi-tag Biotinylated Recombinant Human PD-L1/B7-H1 Display Comparable Bioactivity.

Bio-Techne offers a huge selection of avi-tag and amine-biotinylated proteins to fit your research needs.

https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-fc-chimera-protein-cf_156-b7?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-fc-chimera-avi-tag-protein-cf_avi156?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-fc-chimera-avi-tag-protein-cf_avi156?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/elisa-kits/human-il-2-quantikine-elisa-kit_d2050?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/p/elisa-kits/human-il-2-quantikine-elisa-kit_d2050?pdfSource=true_recombinany-protein-brochure

11

Demonstration of the Utility of Fluorokines for Evaluating CAR Expression. (A) CAR-T cell therapy is based on the principle that T cells removed from a patient or donor can be genetically engineered to express a specific chimeric antigen receptor (CAR). Once

these CAR-T cells are infused back into the patient, the CAR will bind to its specific target

antigen on the surface of the patient’s tumor cells, activating the T cells, and allowing them to

attack and destroy the tumor cells. (B) T cells expressing the CAR can be directly stained using a Fluorokine (fluorescent target antigen) and the percentage of CAR-expressing cells can be

detected by flow cytometry. (C) CD4+CD8+ T cells were transduced with a hCD19-CAR (left) or not transduced (right). Cells were stained with a PE-Cy7-CD4 and CD19 Fc Chimera Atto 488

Protein (Catalog # ATJ9269), and detected by flow cytometry.

Learn More //

Scan the QR Code or Visit:

bio-techne.com/reagents/

proteins/fluorokines-fluorescent-

labeled-proteins

What are Bio-Techne Fluorokines™ and how can I use them for my research? Flouorkines™ are fluorescent-labeled

recombinant proteins that are used to

directly stain and detect target molecules

using flow cytometry. We offer a line

of fluorescent CAR targets that can be

used in flow cytometry and offer greater

specificity than antibodies or Ig-labeling

reagents. Fluorescent-labeled Covid

targets and immune checkpoint targets

are also available. These reagents

undergo rigorous testing to ensure

consistent labeling across lots.

A. B.

Target antigen- specific CAR

CAR-T Cell

Target antigen

Tumor Cell

ACTIVATION, CYTOLYTIC ACTIVITY,

CYTOKINE SECRETION, PROLIFERATION

CAR-T Cell

Fluorescent-labeled target antigen

Target antigen specific CAR

C.

10-3 1030 104 105

CD4

rh C

D 1

9

rhCD19-CAR-T cells

105

104

103

0

CD4

Non-transduced T cells

105

104

103

0

10-3 1030 104 105

Try our Fluorokines™

for your CAR T, SARS-

CoV-2, and immune

checkpoint research.

https://www.bio-techne.com/p/proteins-enzymes/recombinant-human-cd19-protein-atto-488-conjugate_atj9269?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/reagents/proteins/fluorokines-fluorescent-labeled-proteins?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/reagents/proteins/fluorokines-fluorescent-labeled-proteins?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/reagents/proteins/fluorokines-fluorescent-labeled-proteins?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/reagents/proteins/fluorokines-fluorescent-labeled-proteins?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/reagents/proteins/fluorokines-fluorescent-labeled-proteins?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/reagents/proteins/fluorokines-fluorescent-labeled-proteins?pdfSource=true_recombinany-protein-brochure https://www.bio-techne.com/reagents/proteins/fluorokines-fluorescent-labeled-proteins?pdfSource=true_recombinany-protein-brochure

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